Glucocorticoid receptor in clonal osteosarcoma cell lines: A novel system for investigating bone active hormones

M. R. Haussler; S. C. Manolagas; L. J. Deftos

doi:10.1016/S0006-291X(80)80231-2

Glucocorticoid receptor in clonal osteosarcoma cell lines: A novel system for investigating bone active hormones

M. R. Haussler
, S. C. Manolagas
, L. J. Deftos

COM Phx Basic Medical Sci

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Cytosol from cultured osteogenic sarcoma cells contains a high affinity (K_d=1.4×10^-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.

Original language	English (US)
Pages (from-to)	373-380
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/S0006-291X(80)80231-2
State	Published - May 14 1980

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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@article{ae2f096083a144e39e0b99c01ddb6408,

title = "Glucocorticoid receptor in clonal osteosarcoma cell lines: A novel system for investigating bone active hormones",

abstract = "Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.",

author = "Haussler, \{M. R.\} and Manolagas, \{S. C.\} and Deftos, \{L. J.\}",

note = "Funding Information: The authors wish to thank M. Larsen, M. LaFrance and D. Meler for excellent technical assistance. This research was supported by the National Institutes of Health, the American Cancer Society, the Veterans Administration and the Foundation Alexander Onassis. MRH is a faculty scholar of the Josiah Macy, Jr. Foundation.",

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doi = "10.1016/S0006-291X(80)80231-2",

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TY - JOUR

T1 - Glucocorticoid receptor in clonal osteosarcoma cell lines

T2 - A novel system for investigating bone active hormones

AU - Haussler, M. R.

AU - Manolagas, S. C.

AU - Deftos, L. J.

N1 - Funding Information: The authors wish to thank M. Larsen, M. LaFrance and D. Meler for excellent technical assistance. This research was supported by the National Institutes of Health, the American Cancer Society, the Veterans Administration and the Foundation Alexander Onassis. MRH is a faculty scholar of the Josiah Macy, Jr. Foundation.

PY - 1980/5/14

Y1 - 1980/5/14

N2 - Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.

AB - Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.

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UR - https://www.scopus.com/pages/publications/0019324194#tab=citedBy

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AN - SCOPUS:0019324194

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JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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ER -

Glucocorticoid receptor in clonal osteosarcoma cell lines: A novel system for investigating bone active hormones

Abstract

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