TY - JOUR
T1 - Glucocorticoid receptor in clonal osteosarcoma cell lines
T2 - A novel system for investigating bone active hormones
AU - Haussler, M. R.
AU - Manolagas, S. C.
AU - Deftos, L. J.
N1 - Funding Information:
The authors wish to thank M. Larsen, M. LaFrance and D. Meler for excellent technical assistance. This research was supported by the National Institutes of Health, the American Cancer Society, the Veterans Administration and the Foundation Alexander Onassis. MRH is a faculty scholar of the Josiah Macy, Jr. Foundation.
PY - 1980/5/14
Y1 - 1980/5/14
N2 - Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.
AB - Cytosol from cultured osteogenic sarcoma cells contains a high affinity (Kd=1.4×10-9M) triamcinolone(TRM) binding component which adsorbs to DNA-cellulose. The TRM binding molecule sediments at 4S in 0.3M KC1-sucrose gradients, but appears as a 6-7S species when sodium molybdate is present. The concentration of TRM binder is 100fmol/mg cytosol protein (∼ 60,000 receptors/cell) and it displays the classical hierarchy of glucocorticoid potency in competition experiments. Thus, osteogenic sarcoma possesses a typical glucocorticoid receptor. This osteoblast-like malignant bone cell should serve as a model for studying the actions and interactions of glucocorticoids, 1,25-dihydroxyvitamin D and other bone active hormones.
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U2 - 10.1016/S0006-291X(80)80231-2
DO - 10.1016/S0006-291X(80)80231-2
M3 - Article
C2 - 6930269
AN - SCOPUS:0019324194
SN - 0006-291X
VL - 94
SP - 373
EP - 380
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -