Abstract
Background: Electronic-cigarette (e-cig) usage, particularly in the youth population, is a growing concern. It is known that e-cig causes endothelial dysfunction, which is a risk factor for the development of cardiovascular diseases; however, the mechanisms involved remain unclear. We hypothesized that long noncoding RNAs (lncRNAs) may play a role in e-cig-induced endothelial dysfunction. Methods: Here, we identified lncRNAs that are dysregulated in human induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) following 24 h of e-cig aerosol extract treatment via microarray analysis. We performed Gene Ontology and Kyoto Encyclopedia of Genes and Genome (KEGG) pathway analyses of the dysregulated mRNAs following e-cig exposure and constructed co-expression networks of the top 5 upregulated lncRNAs and the top 5 downregulated lncRNAs and the mRNAs that are correlated with them. Furthermore, the functional effects of knocking down lncRNA lung cancer-associated transcript 1 (LUCAT1) on EC phenotypes were determined as it was one of the significantly upregulated lncRNAs following e-cig exposure based on our profiling. Results: 183 lncRNAs and 132 mRNAs were found to be upregulated, whereas 297 lncRNAs and 413 mRNAs were found to be downregulated after e-cig exposure. We also observed that e-cig caused dysregulation of endothelial metabolism resulting in increased FAO activity, higher mitochondrial membrane potential, and decreased glucose uptake and glycolysis. These results suggest that e-cig alters EC metabolism by increasing FAO to compensate for energy deficiency in ECs. Finally, the knockdown of LUCAT1 prevented e-cig-induced EC dysfunction by maintaining vascular barrier, reducing reactive oxygen species level, and increasing migration capacity. Conclusion: This study identifies an expression profile of differentially expressed lncRNAs and several potential regulators and pathways in ECs exposed to e-cig, which provide insights into the regulation of lncRNAs and mRNAs and the role of lncRNA and mRNA networks in ECs associated e-cig exposure.
Original language | English (US) |
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Article number | 593 |
Journal | Stem Cell Research and Therapy |
Volume | 12 |
Issue number | 1 |
DOIs | |
State | Published - Dec 2021 |
Externally published | Yes |
Keywords
- e-cigarettes
- endothelial dysfunction
- fatty acid oxidation
- iPSC-ECs
- lncRNAs
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Molecular Medicine
- Biochemistry, Genetics and Molecular Biology (miscellaneous)
- Cell Biology
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Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract
Le, H. H. T. (Creator), Liu, C.-W. (Creator), Denaro, P. (Creator), Jousma, J. (Creator), Shao, N.-Y. (Creator), Rahman, I. (Creator) & Lee, W. H. (Creator), figshare, 2021
DOI: 10.6084/m9.figshare.c.5737453.v1, https://springernature.figshare.com/collections/Genome-wide_differential_expression_profiling_of_lncRNAs_and_mRNAs_in_human_induced_pluripotent_stem_cell-derived_endothelial_cells_exposed_to_e-cigarette_extract/5737453/1
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Genome-wide differential expression profiling of lncRNAs and mRNAs in human induced pluripotent stem cell-derived endothelial cells exposed to e-cigarette extract
Le, H. H. T. (Creator), Liu, C.-W. (Creator), Denaro, P. (Creator), Jousma, J. (Creator), Shao, N.-Y. (Creator), Rahman, I. (Creator) & Lee, W. H. (Creator), figshare, 2021
DOI: 10.6084/m9.figshare.c.5737453, https://springernature.figshare.com/collections/Genome-wide_differential_expression_profiling_of_lncRNAs_and_mRNAs_in_human_induced_pluripotent_stem_cell-derived_endothelial_cells_exposed_to_e-cigarette_extract/5737453
Dataset