Genome-wide association study of asthma, total IgE, and lung function in a cohort of Peruvian children

Ayobami T. Akenroye, Tonya Brunetti, Karina Romero, Michelle Daya, Kanika Kanchan, Gautam Shankar, Sameer Chavan, Meher Preethi Boorgula, Elizabeth A. Ampleford, Héllen Freitas Fonseca, Gregory A. Hawkins, Helena Mariana Pitangueira Teixeira, Monica Campbell, Nicholas Rafaels, Alexandra Winters, Eugene R. Bleecker, Alvaro A. Cruz, Mauricio L. Barreto, Deborah A. Meyers, Victor E. OrtegaCamila A. Figueiredo, Kathleen C. Barnes, William Checkley, Nadia N. Hansel, Rasika A. Mathias

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Background: Genetic ancestry plays a role in asthma health disparities. Objective: Our aim was to evaluate the impact of ancestry on and identify genetic variants associated with asthma, total serum IgE level, and lung function. Methods: A total of 436 Peruvian children (aged 9-19 years) with asthma and 291 without asthma were genotyped by using the Illumina Multi-Ethnic Global Array. Genome-wide proportions of indigenous ancestry populations from continental America (NAT) and European ancestry from the Iberian populations in Spain (IBS) were estimated by using ADMIXTURE. We assessed the relationship between ancestry and the phenotypes and performed a genome-wide association study. Results: The mean ancestry proportions were 84.7% NAT (case patients, 84.2%; controls, 85.4%) and 15.3% IBS (15.8%; 14.6%). With adjustment for asthma, NAT was associated with higher total serum IgE levels (P <.001) and IBS was associated with lower total serum IgE levels (P <.001). NAT was associated with higher FEV1 percent predicted values (P <.001), whereas IBS was associated with lower FEV1 values in the controls but not in the case patients. The HLA-DR/DQ region on chromosome 6 (Chr6) was strongly associated with total serum IgE (rs3135348; P = 3.438 × 10–10) and was independent of an association with the haplotype HLA-DQA1∼HLA-DQB1:04.01∼04.02 (P = 1.55 × 10–05). For lung function, we identified a locus (rs4410198; P = 5.536 × 10–11) mapping to Chr19, near a cluster of zinc finger interacting genes that colocalizes to the long noncoding RNA CTD-2537I9.5. This novel locus was replicated in an independent sample of pediatric case patients with asthma with similar admixture from Brazil (P =.005). Conclusion: This study confirms the role of HLA in atopy, and identifies a novel locus mapping to a long noncoding RNA for lung function that may be specific to children with NAT.

Original languageEnglish (US)
Pages (from-to)1493-1504
Number of pages12
JournalJournal of Allergy and Clinical Immunology
Volume148
Issue number6
DOIs
StatePublished - Dec 2021

Keywords

  • Asthma
  • Peru
  • admixture
  • allergy
  • ancestry
  • genome wide association analyses
  • immunoglobulin E
  • lung function

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Genome-wide association study of asthma, total IgE, and lung function in a cohort of Peruvian children'. Together they form a unique fingerprint.

Cite this