Abstract
In the past several decades the budding yeast Saccharomyces cerevisiae has emerged as a prominent model for aging research. The creation of a single-gene deletion collection covering the majority of open reading frames in the yeast genome and advances in genomic technologies have opened yeast research to genome-scale screens for a variety of phenotypes. A number of screens have been performed looking for genes that modify secondary age-associated phenotypes such as stress resistance or growth rate. More recently, moderate-throughput methods for measuring replicative life span and high-throughput methods for measuring chronological life span have allowed for the first unbiased screens aimed at directly identifying genes involved in determining yeast longevity. In this chapter we discuss large-scale life span studies performed in yeast and their implications for research related to the basic biology of aging.
Original language | English (US) |
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Pages (from-to) | 251-289 |
Number of pages | 39 |
Journal | Sub-cellular biochemistry |
Volume | 57 |
DOIs | |
State | Published - 2012 |
Externally published | Yes |
Keywords
- Acetic acid
- Apoptosis
- Asymmetric segregation
- Chronological life span
- Counter flow centrifugation elutriation (CCE)
- Dietary restriction (DR)
- Genome-wide
- Genomics
- High-throughput
- Loss of heterozygosity (LOH)
- Metabolomics
- Microarrays
- Mitochondria
- Mitochondrial back-signaling
- Mother Enrichment Program (MEP)
- Oxidative damage
- Proteomics
- Replicative life span
- Retrograde response
- Ribosomal DNA (rDNA)
- Sirtuins
- Target of rapamycin (TOR) signaling
- Translation
- Worms
- Yeast Outgrowth Data Analysis (YODA)
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology
- Cancer Research