Genome-wide analysis of yeast aging

George L. Sutphin, Brady A. Olsen, Brian K. Kennedy, Matt Kaeberlein

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

In the past several decades the budding yeast Saccharomyces cerevisiae has emerged as a prominent model for aging research. The creation of a single-gene deletion collection covering the majority of open reading frames in the yeast genome and advances in genomic technologies have opened yeast research to genome-scale screens for a variety of phenotypes. A number of screens have been performed looking for genes that modify secondary age-associated phenotypes such as stress resistance or growth rate. More recently, moderate-throughput methods for measuring replicative life span and high-throughput methods for measuring chronological life span have allowed for the first unbiased screens aimed at directly identifying genes involved in determining yeast longevity. In this chapter we discuss large-scale life span studies performed in yeast and their implications for research related to the basic biology of aging.

Original languageEnglish (US)
Pages (from-to)251-289
Number of pages39
JournalSub-cellular biochemistry
Volume57
DOIs
StatePublished - 2012
Externally publishedYes

Keywords

  • Acetic acid
  • Apoptosis
  • Asymmetric segregation
  • Chronological life span
  • Counter flow centrifugation elutriation (CCE)
  • Dietary restriction (DR)
  • Genome-wide
  • Genomics
  • High-throughput
  • Loss of heterozygosity (LOH)
  • Metabolomics
  • Microarrays
  • Mitochondria
  • Mitochondrial back-signaling
  • Mother Enrichment Program (MEP)
  • Oxidative damage
  • Proteomics
  • Replicative life span
  • Retrograde response
  • Ribosomal DNA (rDNA)
  • Sirtuins
  • Target of rapamycin (TOR) signaling
  • Translation
  • Worms
  • Yeast Outgrowth Data Analysis (YODA)

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology
  • Cancer Research

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