Genome-wide analysis of G-quadruplex in Spodoptera frugiperda

Zhongyuan Deng, Yudong Ren, Lina Guo, Xingcheng Xie, Lixiang Wang, Xianchun Li

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Spodoptera frugiperda (Lepidoptera: Noctuidae) is a globally distributed lepidopteran crop pest that has developed resistance to most insecticides. The G-quadruplex (G4) is a secondary structure in the genome enriched in the promoters for regulating gene expression. However, little is known about G4 in S. frugiperda, especially whether G4 is involved in insecticide resistance and pest control. In this study, 387,875 G4 motifs in the whole genome of S. frugiperda were identified by bioinformatics prediction. We found that 66.90 % of theseG4 structures were located in genic regions and highly enriched in the upstream regions of start codons. Functional and pathway analyses showed that the genes with G4 enriched in promoter regions participate in several metabolic processes. Further analyses showed that G4 structures occurred more frequently in the promoters of P450 and CarE gene families. It was also investigated that G4 ligand N-methyl mesoporphyrin IX (NMM) decreased P450 protein activity in larval midgut tissue. Cytotoxicity and bioassay results revealed that NMM and pesticides had synergistic effects on toxicity. In conclusion, our findings suggest that G4 motif could be a new potential target for pest control.

Original languageEnglish (US)
Pages (from-to)840-852
Number of pages13
JournalInternational Journal of Biological Macromolecules
Volume226
DOIs
StatePublished - Jan 31 2023

Keywords

  • DNA secondary structure
  • G-quadruplex
  • NMM
  • Pest control
  • Spodoptera frugiperda

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

Fingerprint

Dive into the research topics of 'Genome-wide analysis of G-quadruplex in Spodoptera frugiperda'. Together they form a unique fingerprint.

Cite this