Genome sequence of the tsetse fly (Glossina morsitans ): Vector of African trypanosomiasis

Junichi Watanabe; Masahira Hattori; Matthew Berriman; Michael J. Lehane; Neil Hall; Philippe Solano; Serap Aksoy; Winston Hide; Yeya Touré; Geoffrey M. Attardo; Alistair C. Darby; Atsushi Toyoda; Christiane Hertz-Fowler; Denis M. Larkin; James A. Cotton; Mandy J. Sanders; Martin T. Swain; Michael A. Quail; Noboru Inoue; Sophie Ravel; Todd D. Taylor; Tulika P. Srivastava; Vineet Sharma; Wesley Warren; Richard K. Wilson; Yutaka Suzuki; Daniel Lawson; Daniel S.T. Hughes; Karyn Megy; Daniel K. Masiga; Paul O. Mireji; Immo A. Hansen; Jan Van Den Abbeele; Joshua B. Benoit; Kostas Bourtzis; George F.O. Obiero; Hugh M. Robertson; Jeffery W. Jones; Jing Jiang Zhou; Linda M. Field; Markus Friedrich; Steven R.G. Nyanjom; Erich L. Telleria; Guy Caljon; José M.C. Ribeiro; Alvaro Acosta-Serrano; Cher Pheng Ooi; Clair Rose; David P. Price; Lee R. Haines; Alan Christoffels; Cheolho Sim; Daphne Q.D. Pham; David L. Denlinger; Dawn L. Geiser; Irene A. Omedo; Joy J. Winzerling; Justin T. Peyton; Kevin K. Marucha; Mario Jonas; Megan E. Meuti; Neil D. Rawlings; Qirui Zhang; Rosaline W. Macharia; Veronika Michalkova; Zahra Jalali Sefid Dashti; Aaron A. Baumann; Gerd Gäde; Heather G. Marco; Jelle Caers; Liliane Schoofs; Michael A. Riehle; Wanqi Hu; Zhijian Tu; Aaron M. Tarone; Anna R. Malacrida; Caleb K. Kibet; Francesca Scolari; Jacobus J.O. Koekemoer; Judith Willis; Ludvik M. Gomulski; Marco Falchetto; Maxwell J. Scott; Shuhua Fu; Sing Hoi Sze; Thiago Luiz; Brian Weiss; Deirdre P. Walshe; Jingwen Wang; Mark Wamalwa; Sarah Mwangi; Urvashi N. Ramphul; Anna K. Snyder; Corey L. Brelsfoard; Gavin H. Thomas; George Tsiamis; Peter Arensburger; Rita V.M. Rio; Sandy J. Macdonald; Sumir Panji; Adele Kruger; Alia Benkahla; Apollo S.P. Balyeidhusa; Atway Msangi; Chinyere K. Okoro; Dawn Stephens; Eleanor J. Stanley; Feziwe Mpondo; Florence Wamwiri; Furaha Mramba; Geoffrey Siwo; George Githinji; Gordon Harkins; Grace Murilla; Heikki Lehväslaiho; Imna Malele; Joanna E. Auma; Johnson K. Kinyua; Johnson Ouma; Loyce Okedi; Lucien Manga; Martin Aslett; Mathurin Koffi; Michael W. Gaunt; Mmule Makgamathe; Nicola Mulder; Oliver Manangwa; Patrick P. Abila; Patrick Wincker; Richard Gregory; Rosemary Bateta; Ryuichi Sakate; Sheila Ommeh; Stella Lehane; Tadashi Imanishi; Victor C. Osamor; Yoshihiro Kawahara

doi:10.1126/science.1249656

Genome sequence of the tsetse fly (Glossina morsitans ): Vector of African trypanosomiasis

Junichi Watanabe, Masahira Hattori, Matthew Berriman, Michael J. Lehane, Neil Hall, Philippe Solano, Serap Aksoy, Winston Hide, Yeya Touré, Geoffrey M. Attardo, Alistair C. Darby, Atsushi Toyoda, Christiane Hertz-Fowler, Denis M. Larkin, James A. Cotton, Mandy J. Sanders, Martin T. Swain, Michael A. Quail, Noboru Inoue, Sophie RavelTodd D. Taylor, Tulika P. Srivastava, Vineet Sharma, Wesley Warren, Richard K. Wilson, Yutaka Suzuki, Daniel Lawson, Daniel S.T. Hughes, Karyn Megy, Daniel K. Masiga, Paul O. Mireji, Immo A. Hansen, Jan Van Den Abbeele, Joshua B. Benoit, Kostas Bourtzis, George F.O. Obiero, Hugh M. Robertson, Jeffery W. Jones, Jing Jiang Zhou, Linda M. Field, Markus Friedrich, Steven R.G. Nyanjom, Erich L. Telleria, Guy Caljon, José M.C. Ribeiro, Alvaro Acosta-Serrano, Cher Pheng Ooi, Clair Rose, David P. Price, Lee R. Haines, Alan Christoffels, Cheolho Sim, Daphne Q.D. Pham, David L. Denlinger, Dawn L. Geiser, Irene A. Omedo, Joy J. Winzerling, Justin T. Peyton, Kevin K. Marucha, Mario Jonas, Megan E. Meuti, Neil D. Rawlings, Qirui Zhang, Rosaline W. Macharia, Veronika Michalkova, Zahra Jalali Sefid Dashti, Aaron A. Baumann, Gerd Gäde, Heather G. Marco, Jelle Caers, Liliane Schoofs, Michael A. Riehle, Wanqi Hu, Zhijian Tu, Aaron M. Tarone, Anna R. Malacrida, Caleb K. Kibet, Francesca Scolari, Jacobus J.O. Koekemoer, Judith Willis, Ludvik M. Gomulski, Marco Falchetto, Maxwell J. Scott, Shuhua Fu, Sing Hoi Sze, Thiago Luiz, Brian Weiss, Deirdre P. Walshe, Jingwen Wang, Mark Wamalwa, Sarah Mwangi, Urvashi N. Ramphul, Anna K. Snyder, Corey L. Brelsfoard, Gavin H. Thomas, George Tsiamis, Peter Arensburger, Rita V.M. Rio, Sandy J. Macdonald, Sumir Panji, Adele Kruger, Alia Benkahla, Apollo S.P. Balyeidhusa, Atway Msangi, Chinyere K. Okoro, Dawn Stephens, Eleanor J. Stanley, Feziwe Mpondo, Florence Wamwiri, Furaha Mramba, Geoffrey Siwo, George Githinji, Gordon Harkins, Grace Murilla, Heikki Lehväslaiho, Imna Malele, Joanna E. Auma, Johnson K. Kinyua, Johnson Ouma, Loyce Okedi, Lucien Manga, Martin Aslett, Mathurin Koffi, Michael W. Gaunt, Mmule Makgamathe, Nicola Mulder, Oliver Manangwa, Patrick P. Abila, Patrick Wincker, Richard Gregory, Rosemary Bateta, Ryuichi Sakate, Sheila Ommeh, Stella Lehane, Tadashi Imanishi, Victor C. Osamor, Yoshihiro Kawahara

Research output: Contribution to journal › Article › peer-review

193 Scopus citations

Abstract

Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes of adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between tsetse and other disease vectors include obligate microbial symbioses, viviparous reproduction, and lactation. Here, we describe the sequence and annotation of the 366-megabase Glossina morsitans morsitans genome. Analysis of the genome and the 12,308 predicted protein-encoding genes led to multiple discoveries, including chromosomal integrations of bacterial (Wolbachia) genome sequences, a family of lactation-specific proteins, reduced complement of host pathogen recognition proteins, and reduced olfaction/chemosensory associated genes. These genome data provide a foundation for research into trypanosomiasis prevention and yield important insights with broad implications for multiple aspects of tsetse biology.

Original language	English (US)
Pages (from-to)	380-386
Number of pages	7
Journal	Science
Volume	344
Issue number	6182
DOIs	https://doi.org/10.1126/science.1249656
State	Published - 2014

ASJC Scopus subject areas

General

Access to Document

10.1126/science.1249656

Cite this

Watanabe, J., Hattori, M., Berriman, M., Lehane, M. J., Hall, N., Solano, P., Aksoy, S., Hide, W., Touré, Y., Attardo, G. M., Darby, A. C., Toyoda, A., Hertz-Fowler, C., Larkin, D. M., Cotton, J. A., Sanders, M. J., Swain, M. T., Quail, M. A., Inoue, N., ... Kawahara, Y. (2014). Genome sequence of the tsetse fly (Glossina morsitans ): Vector of African trypanosomiasis. Science, 344(6182), 380-386. https://doi.org/10.1126/science.1249656

Watanabe, J, Hattori, M, Berriman, M, Lehane, MJ, Hall, N, Solano, P, Aksoy, S, Hide, W, Touré, Y, Attardo, GM, Darby, AC, Toyoda, A, Hertz-Fowler, C, Larkin, DM, Cotton, JA, Sanders, MJ, Swain, MT, Quail, MA, Inoue, N, Ravel, S, Taylor, TD, Srivastava, TP, Sharma, V, Warren, W, Wilson, RK, Suzuki, Y, Lawson, D, Hughes, DST, Megy, K, Masiga, DK, Mireji, PO, Hansen, IA, Van Den Abbeele, J, Benoit, JB, Bourtzis, K, Obiero, GFO, Robertson, HM, Jones, JW, Zhou, JJ, Field, LM, Friedrich, M, Nyanjom, SRG, Telleria, EL, Caljon, G, Ribeiro, JMC, Acosta-Serrano, A, Ooi, CP, Rose, C, Price, DP, Haines, LR, Christoffels, A, Sim, C, Pham, DQD, Denlinger, DL, Geiser, DL, Omedo, IA, Winzerling, JJ, Peyton, JT, Marucha, KK, Jonas, M, Meuti, ME, Rawlings, ND, Zhang, Q, Macharia, RW, Michalkova, V, Dashti, ZJS, Baumann, AA, Gäde, G, Marco, HG, Caers, J, Schoofs, L, Riehle, MA, Hu, W, Tu, Z, Tarone, AM, Malacrida, AR, Kibet, CK, Scolari, F, Koekemoer, JJO, Willis, J, Gomulski, LM, Falchetto, M, Scott, MJ, Fu, S, Sze, SH, Luiz, T, Weiss, B, Walshe, DP, Wang, J, Wamalwa, M, Mwangi, S, Ramphul, UN, Snyder, AK, Brelsfoard, CL, Thomas, GH, Tsiamis, G, Arensburger, P, Rio, RVM, Macdonald, SJ, Panji, S, Kruger, A, Benkahla, A, Balyeidhusa, ASP, Msangi, A, Okoro, CK, Stephens, D, Stanley, EJ, Mpondo, F, Wamwiri, F, Mramba, F, Siwo, G, Githinji, G, Harkins, G, Murilla, G, Lehväslaiho, H, Malele, I, Auma, JE, Kinyua, JK, Ouma, J, Okedi, L, Manga, L, Aslett, M, Koffi, M, Gaunt, MW, Makgamathe, M, Mulder, N, Manangwa, O, Abila, PP, Wincker, P, Gregory, R, Bateta, R, Sakate, R, Ommeh, S, Lehane, S, Imanishi, T, Osamor, VC & Kawahara, Y 2014, 'Genome sequence of the tsetse fly (Glossina morsitans ): Vector of African trypanosomiasis', Science, vol. 344, no. 6182, pp. 380-386. https://doi.org/10.1126/science.1249656

@article{ca6ccd5c4281454f9845209ec0004892,

title = "Genome sequence of the tsetse fly (Glossina morsitans ): Vector of African trypanosomiasis",

abstract = "Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes of adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between tsetse and other disease vectors include obligate microbial symbioses, viviparous reproduction, and lactation. Here, we describe the sequence and annotation of the 366-megabase Glossina morsitans morsitans genome. Analysis of the genome and the 12,308 predicted protein-encoding genes led to multiple discoveries, including chromosomal integrations of bacterial (Wolbachia) genome sequences, a family of lactation-specific proteins, reduced complement of host pathogen recognition proteins, and reduced olfaction/chemosensory associated genes. These genome data provide a foundation for research into trypanosomiasis prevention and yield important insights with broad implications for multiple aspects of tsetse biology.",

author = "Junichi Watanabe and Masahira Hattori and Matthew Berriman and Lehane, {Michael J.} and Neil Hall and Philippe Solano and Serap Aksoy and Winston Hide and Yeya Tour{\'e} and Attardo, {Geoffrey M.} and Darby, {Alistair C.} and Atsushi Toyoda and Christiane Hertz-Fowler and Larkin, {Denis M.} and Cotton, {James A.} and Sanders, {Mandy J.} and Swain, {Martin T.} and Quail, {Michael A.} and Noboru Inoue and Sophie Ravel and Taylor, {Todd D.} and Srivastava, {Tulika P.} and Vineet Sharma and Wesley Warren and Wilson, {Richard K.} and Yutaka Suzuki and Daniel Lawson and Hughes, {Daniel S.T.} and Karyn Megy and Masiga, {Daniel K.} and Mireji, {Paul O.} and Hansen, {Immo A.} and {Van Den Abbeele}, Jan and Benoit, {Joshua B.} and Kostas Bourtzis and Obiero, {George F.O.} and Robertson, {Hugh M.} and Jones, {Jeffery W.} and Zhou, {Jing Jiang} and Field, {Linda M.} and Markus Friedrich and Nyanjom, {Steven R.G.} and Telleria, {Erich L.} and Guy Caljon and Ribeiro, {Jos{\'e} M.C.} and Alvaro Acosta-Serrano and Ooi, {Cher Pheng} and Clair Rose and Price, {David P.} and Haines, {Lee R.} and Alan Christoffels and Cheolho Sim and Pham, {Daphne Q.D.} and Denlinger, {David L.} and Geiser, {Dawn L.} and Omedo, {Irene A.} and Winzerling, {Joy J.} and Peyton, {Justin T.} and Marucha, {Kevin K.} and Mario Jonas and Meuti, {Megan E.} and Rawlings, {Neil D.} and Qirui Zhang and Macharia, {Rosaline W.} and Veronika Michalkova and Dashti, {Zahra Jalali Sefid} and Baumann, {Aaron A.} and Gerd G{\"a}de and Marco, {Heather G.} and Jelle Caers and Liliane Schoofs and Riehle, {Michael A.} and Wanqi Hu and Zhijian Tu and Tarone, {Aaron M.} and Malacrida, {Anna R.} and Kibet, {Caleb K.} and Francesca Scolari and Koekemoer, {Jacobus J.O.} and Judith Willis and Gomulski, {Ludvik M.} and Marco Falchetto and Scott, {Maxwell J.} and Shuhua Fu and Sze, {Sing Hoi} and Thiago Luiz and Brian Weiss and Walshe, {Deirdre P.} and Jingwen Wang and Mark Wamalwa and Sarah Mwangi and Ramphul, {Urvashi N.} and Snyder, {Anna K.} and Brelsfoard, {Corey L.} and Thomas, {Gavin H.} and George Tsiamis and Peter Arensburger and Rio, {Rita V.M.} and Macdonald, {Sandy J.} and Sumir Panji and Adele Kruger and Alia Benkahla and Balyeidhusa, {Apollo S.P.} and Atway Msangi and Okoro, {Chinyere K.} and Dawn Stephens and Stanley, {Eleanor J.} and Feziwe Mpondo and Florence Wamwiri and Furaha Mramba and Geoffrey Siwo and George Githinji and Gordon Harkins and Grace Murilla and Heikki Lehv{\"a}slaiho and Imna Malele and Auma, {Joanna E.} and Kinyua, {Johnson K.} and Johnson Ouma and Loyce Okedi and Lucien Manga and Martin Aslett and Mathurin Koffi and Gaunt, {Michael W.} and Mmule Makgamathe and Nicola Mulder and Oliver Manangwa and Abila, {Patrick P.} and Patrick Wincker and Richard Gregory and Rosemary Bateta and Ryuichi Sakate and Sheila Ommeh and Stella Lehane and Tadashi Imanishi and Osamor, {Victor C.} and Yoshihiro Kawahara",

year = "2014",

doi = "10.1126/science.1249656",

language = "English (US)",

volume = "344",

pages = "380--386",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6182",

}

TY - JOUR

T1 - Genome sequence of the tsetse fly (Glossina morsitans )

T2 - Vector of African trypanosomiasis

AU - Watanabe, Junichi

AU - Hattori, Masahira

AU - Berriman, Matthew

AU - Lehane, Michael J.

AU - Hall, Neil

AU - Solano, Philippe

AU - Aksoy, Serap

AU - Hide, Winston

AU - Touré, Yeya

AU - Attardo, Geoffrey M.

AU - Darby, Alistair C.

AU - Toyoda, Atsushi

AU - Hertz-Fowler, Christiane

AU - Larkin, Denis M.

AU - Cotton, James A.

AU - Sanders, Mandy J.

AU - Swain, Martin T.

AU - Quail, Michael A.

AU - Inoue, Noboru

AU - Ravel, Sophie

AU - Taylor, Todd D.

AU - Srivastava, Tulika P.

AU - Sharma, Vineet

AU - Warren, Wesley

AU - Wilson, Richard K.

AU - Suzuki, Yutaka

AU - Lawson, Daniel

AU - Hughes, Daniel S.T.

AU - Megy, Karyn

AU - Masiga, Daniel K.

AU - Mireji, Paul O.

AU - Hansen, Immo A.

AU - Van Den Abbeele, Jan

AU - Benoit, Joshua B.

AU - Bourtzis, Kostas

AU - Obiero, George F.O.

AU - Robertson, Hugh M.

AU - Jones, Jeffery W.

AU - Zhou, Jing Jiang

AU - Field, Linda M.

AU - Friedrich, Markus

AU - Nyanjom, Steven R.G.

AU - Telleria, Erich L.

AU - Caljon, Guy

AU - Ribeiro, José M.C.

AU - Acosta-Serrano, Alvaro

AU - Ooi, Cher Pheng

AU - Rose, Clair

AU - Price, David P.

AU - Haines, Lee R.

AU - Christoffels, Alan

AU - Sim, Cheolho

AU - Pham, Daphne Q.D.

AU - Denlinger, David L.

AU - Geiser, Dawn L.

AU - Omedo, Irene A.

AU - Winzerling, Joy J.

AU - Peyton, Justin T.

AU - Marucha, Kevin K.

AU - Jonas, Mario

AU - Meuti, Megan E.

AU - Rawlings, Neil D.

AU - Zhang, Qirui

AU - Macharia, Rosaline W.

AU - Michalkova, Veronika

AU - Dashti, Zahra Jalali Sefid

AU - Baumann, Aaron A.

AU - Gäde, Gerd

AU - Marco, Heather G.

AU - Caers, Jelle

AU - Schoofs, Liliane

AU - Riehle, Michael A.

AU - Hu, Wanqi

AU - Tu, Zhijian

AU - Tarone, Aaron M.

AU - Malacrida, Anna R.

AU - Kibet, Caleb K.

AU - Scolari, Francesca

AU - Koekemoer, Jacobus J.O.

AU - Willis, Judith

AU - Gomulski, Ludvik M.

AU - Falchetto, Marco

AU - Scott, Maxwell J.

AU - Fu, Shuhua

AU - Sze, Sing Hoi

AU - Luiz, Thiago

AU - Weiss, Brian

AU - Walshe, Deirdre P.

AU - Wang, Jingwen

AU - Wamalwa, Mark

AU - Mwangi, Sarah

AU - Ramphul, Urvashi N.

AU - Snyder, Anna K.

AU - Brelsfoard, Corey L.

AU - Thomas, Gavin H.

AU - Tsiamis, George

AU - Arensburger, Peter

AU - Rio, Rita V.M.

AU - Macdonald, Sandy J.

AU - Panji, Sumir

AU - Kruger, Adele

AU - Benkahla, Alia

AU - Balyeidhusa, Apollo S.P.

AU - Msangi, Atway

AU - Okoro, Chinyere K.

AU - Stephens, Dawn

AU - Stanley, Eleanor J.

AU - Mpondo, Feziwe

AU - Wamwiri, Florence

AU - Mramba, Furaha

AU - Siwo, Geoffrey

AU - Githinji, George

AU - Harkins, Gordon

AU - Murilla, Grace

AU - Lehväslaiho, Heikki

AU - Malele, Imna

AU - Auma, Joanna E.

AU - Kinyua, Johnson K.

AU - Ouma, Johnson

AU - Okedi, Loyce

AU - Manga, Lucien

AU - Aslett, Martin

AU - Koffi, Mathurin

AU - Gaunt, Michael W.

AU - Makgamathe, Mmule

AU - Mulder, Nicola

AU - Manangwa, Oliver

AU - Abila, Patrick P.

AU - Wincker, Patrick

AU - Gregory, Richard

AU - Bateta, Rosemary

AU - Sakate, Ryuichi

AU - Ommeh, Sheila

AU - Lehane, Stella

AU - Imanishi, Tadashi

AU - Osamor, Victor C.

AU - Kawahara, Yoshihiro

PY - 2014

Y1 - 2014

N2 - Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes of adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between tsetse and other disease vectors include obligate microbial symbioses, viviparous reproduction, and lactation. Here, we describe the sequence and annotation of the 366-megabase Glossina morsitans morsitans genome. Analysis of the genome and the 12,308 predicted protein-encoding genes led to multiple discoveries, including chromosomal integrations of bacterial (Wolbachia) genome sequences, a family of lactation-specific proteins, reduced complement of host pathogen recognition proteins, and reduced olfaction/chemosensory associated genes. These genome data provide a foundation for research into trypanosomiasis prevention and yield important insights with broad implications for multiple aspects of tsetse biology.

AB - Tsetse flies are the sole vectors of human African trypanosomiasis throughout sub-Saharan Africa. Both sexes of adult tsetse feed exclusively on blood and contribute to disease transmission. Notable differences between tsetse and other disease vectors include obligate microbial symbioses, viviparous reproduction, and lactation. Here, we describe the sequence and annotation of the 366-megabase Glossina morsitans morsitans genome. Analysis of the genome and the 12,308 predicted protein-encoding genes led to multiple discoveries, including chromosomal integrations of bacterial (Wolbachia) genome sequences, a family of lactation-specific proteins, reduced complement of host pathogen recognition proteins, and reduced olfaction/chemosensory associated genes. These genome data provide a foundation for research into trypanosomiasis prevention and yield important insights with broad implications for multiple aspects of tsetse biology.

UR - http://www.scopus.com/inward/record.url?scp=84899560044&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84899560044&partnerID=8YFLogxK

U2 - 10.1126/science.1249656

DO - 10.1126/science.1249656

M3 - Article

C2 - 24763584

AN - SCOPUS:84899560044

SN - 0036-8075

VL - 344

SP - 380

EP - 386

JO - Science

JF - Science

IS - 6182

ER -

Genome sequence of the tsetse fly (Glossina morsitans ): Vector of African trypanosomiasis

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this