@article{41764ef92fc1468d9857175d4191630b,
title = "Genome-enabled discovery of anthraquinone biosynthesis in Senna tora",
abstract = "Senna tora is a widely used medicinal plant. Its health benefits have been attributed to the large quantity of anthraquinones, but how they are made in plants remains a mystery. To identify the genes responsible for plant anthraquinone biosynthesis, we reveal the genome sequence of S. tora at the chromosome level with 526 Mb (96%) assembled into 13 chromosomes. Comparison among related plant species shows that a chalcone synthase-like (CHS-L) gene family has lineage-specifically and rapidly expanded in S. tora. Combining genomics, transcriptomics, metabolomics, and biochemistry, we identify a CHS-L gene contributing to the biosynthesis of anthraquinones. The S. tora reference genome will accelerate the discovery of biologically active anthraquinone biosynthesis pathways in medicinal plants.",
author = "Kang, {Sang Ho} and Pandey, {Ramesh Prasad} and Lee, {Chang Muk} and Sim, {Joon Soo} and Jeong, {Jin Tae} and Choi, {Beom Soon} and Myunghee Jung and Daniel Ginzburg and Kangmei Zhao and Won, {So Youn} and Oh, {Tae Jin} and Yeisoo Yu and Kim, {Nam Hoon} and Lee, {Ok Ran} and Lee, {Tae Ho} and Puspalata Bashyal and Kim, {Tae Su} and Lee, {Woo Haeng} and Charles Hawkins and Kim, {Chang Kug} and Kim, {Jung Sun} and Ahn, {Byoung Ohg} and Rhee, {Seung Yon} and Sohng, {Jae Kyung}",
note = "Funding Information: We thank the National Institute of Agricultural Sciences (NAS) Genome Sequencing Core facility for its services. We thank DNA Link (Seoul, Korea) for their assistance to generate the PacBio data and contig assembly. We thank Phase Genomics (Seattle, USA) for their assistance in the generation of the Hi-C data and superscaffold assembly. We thank Insilicogen, Inc. for their assistance in genomic structural and functional annotations and evolutionary analysis. We thank Human Metabolome Technologies Inc. (Yamagata, Japan) for their assistance in analyzing the primary metabolites. We thank GnC Bio Co. for their assistance in the generation of BAC libraries. This work was carried out with the support of the National Institute of Agricultural Sciences (Project no. PJ013818) and Cooperative Research Program for Agriculture Science and Technology Development (Project title: National Agricultural Genome Program, Project no. PJ010457), Rural Development Administration, Republic of Korea. Funding for S.Y.R. was provided by the National Institutes of Health (grant no. 1U01GM110699-01A1) and the National Science Foundation (IOS-1546838, IOS-1026003). Publisher Copyright: {\textcopyright} 2020, The Author(s).",
year = "2020",
month = dec,
doi = "10.1038/s41467-020-19681-1",
language = "English (US)",
volume = "11",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}