TY - JOUR
T1 - Genetic Susceptibility of Cultured Shrimp (Penaeus vannamei) to Infectious Hypodermal and Hematopoietic Necrosis Virus andBaculovirus penaei:Possible Relationship with Growth Status and Metabolic Gene Expression
AU - Alcivar-Warren, Acacia
AU - Overstreet, Robin M.
AU - Dhar, Arun K.
AU - Astrofsky, Keith
AU - Carr, William H.
AU - Sweeney, James
AU - Lotz, Jeffrey M.
N1 - Funding Information:
We greatly appreciate the technical support offered by Ms. Maura Faggart and Ms. Julie Gonsalves at Tufts University School of Veterinary Medicine (TUSVM); Ms. Lidia Stuck of Gulf Coast Research Laboratory; and Mr. Charlie Chang, Mr. Ricky Oyama, Ms. Sharon Roddy, and Mr. Robert Kanna at The Oceanic Institute. We thank Dr. Karl Ebert, Dr. Norman Hecht, and Dr. Denise Garcia for providing comments to the manuscript and Dr. Laurel Rhodes for helping with sample collection while she was a veterinary medical student at TUSVM. This study was conducted in cooperation with the U.S. Department of Agriculture, CSREES, Grant 95-38808-1424 to the U.S. Marine Shrimp Farming Program.
PY - 1997/11
Y1 - 1997/11
N2 - Offspring of four crosses (I, II, III, and IV) ofPenaeus vannameifrom known high- and low-growth families were challenged with infectious hypodermal and hematopoetic necrosis virus (IHHNV) andBaculovirus penaei(BP) to compare their susceptibility to these viral agents and examine the genetic component involved in disease resistance or susceptibility. Family crosses were made using broodstock from five families developed by the U.S. Marine Shrimp Farming Program. The prevalence of IHHNV infection was highest in cross I and lowest in cross III. Cross I was developed using male and female broodstock from the low-growth family 1.6, and cross III was developed using a female from the high-growth family 1.3 and a male from the low-growth family 1.6. The prevalence of BP infection at Day 4 was highest (100%) in cross IV, which was developed using a female from the low-growth family 1.4 and a male from the high-growth family 1.5. The reciprocal cross, cross III, had the lowest (68%) prevalence at Day 4 postexposure. Both crosses I and II had 88% prevalence of infection at Day 4. Despite 100% prevalence of BP infection in cross IV at 4 days, animals from this cross and cross II exhibited high survival by Day 18 (85 and 77%). On the other hand, crosses I and III (with 88 and 68% prevalence at Day 4, respectively) showed low survival at Day 18 (19 and 24%). On the basis of prevalence of infection and mortality rates, it was concluded that the susceptibility to BP in penaeid shrimp is governed by the genetic background of the parental crosses. The random amplified polymorphic DNA polymorphisms for crosses I, II, III, and IV, were 43, 45, 53, and 51%, respectively, showing no clear relationship between IHHNV and BP prevalence of infection and levels of nuclear genetic diversity. Though the mtDNA haplotypes in offspring from the different crosses were the same, major differences were observed in both steady-state levels and patterns of expression of the mitochondrial 12s rRNA in offspring obtained at various early developmental stages from each of the four crosses. The possible relationship among disease susceptibility, growth status, and expression of mitochondrial 12s rRNA is discussed in the context of a complex nuclear-cytoplasmic genetic system involved in the regulation of gene expression.
AB - Offspring of four crosses (I, II, III, and IV) ofPenaeus vannameifrom known high- and low-growth families were challenged with infectious hypodermal and hematopoetic necrosis virus (IHHNV) andBaculovirus penaei(BP) to compare their susceptibility to these viral agents and examine the genetic component involved in disease resistance or susceptibility. Family crosses were made using broodstock from five families developed by the U.S. Marine Shrimp Farming Program. The prevalence of IHHNV infection was highest in cross I and lowest in cross III. Cross I was developed using male and female broodstock from the low-growth family 1.6, and cross III was developed using a female from the high-growth family 1.3 and a male from the low-growth family 1.6. The prevalence of BP infection at Day 4 was highest (100%) in cross IV, which was developed using a female from the low-growth family 1.4 and a male from the high-growth family 1.5. The reciprocal cross, cross III, had the lowest (68%) prevalence at Day 4 postexposure. Both crosses I and II had 88% prevalence of infection at Day 4. Despite 100% prevalence of BP infection in cross IV at 4 days, animals from this cross and cross II exhibited high survival by Day 18 (85 and 77%). On the other hand, crosses I and III (with 88 and 68% prevalence at Day 4, respectively) showed low survival at Day 18 (19 and 24%). On the basis of prevalence of infection and mortality rates, it was concluded that the susceptibility to BP in penaeid shrimp is governed by the genetic background of the parental crosses. The random amplified polymorphic DNA polymorphisms for crosses I, II, III, and IV, were 43, 45, 53, and 51%, respectively, showing no clear relationship between IHHNV and BP prevalence of infection and levels of nuclear genetic diversity. Though the mtDNA haplotypes in offspring from the different crosses were the same, major differences were observed in both steady-state levels and patterns of expression of the mitochondrial 12s rRNA in offspring obtained at various early developmental stages from each of the four crosses. The possible relationship among disease susceptibility, growth status, and expression of mitochondrial 12s rRNA is discussed in the context of a complex nuclear-cytoplasmic genetic system involved in the regulation of gene expression.
KW - IHHNV; Baculovirus penaei; Penaeus vannamei; genetic diversity; susceptibility; 12s RNA; mtDNA
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U2 - 10.1006/jipa.1997.4692
DO - 10.1006/jipa.1997.4692
M3 - Article
AN - SCOPUS:0001658111
VL - 70
SP - 190
EP - 197
JO - Journal of Invertebrate Pathology
JF - Journal of Invertebrate Pathology
SN - 0022-2011
IS - 3
ER -