Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019

Arun K. Dhar; Roberto Cruz-Flores; Janet Warg; Mary L. Killian; Andrew Orry; Jorge Ramos; Michelle Garfias; Gregory Lyons

doi:10.3201/eid2802.211874

Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019

Arun K. Dhar, Roberto Cruz-Flores, Janet Warg, Mary L. Killian, Andrew Orry, Jorge Ramos, Michelle Garfias, Gregory Lyons

Animal and Biomedical Sciences, Research

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a nonenveloped, linear, single-stranded DNA virus belonging to the family Parvoviridae and is a World Organisation for Animal Health (OIE)-notifi able crustacean pathogen. During screening of Penaeus vannamei shrimp from 3 commercial shrimp facilities in the United States for a panel of OIE-listed (n = 7) and nonlisted (n = 2) crustacean diseases, shrimp from these facilities tested positive for IHHNV. Nucleotide sequences of PCR amplicons showed 99%-100% similarity to IHHNV isolates from Latin America and Asia. The whole genome of the isolates also showed high similarity to type 2 infectious forms of IHHNV. Phylogenetic analysis using capsid gene and whole-genome sequences demonstrated that the isolates clustered with an IHHNV isolate from Ecuador. The detection of an OIE-listed crustacean pathogen in the United States highlights the need for biosecurity protocols in hatcheries and grow-out ponds to mitigate losses.

Original language	English (US)
Pages (from-to)	373-381
Number of pages	9
Journal	Emerging infectious diseases
Volume	28
Issue number	2
DOIs	https://doi.org/10.3201/eid2802.211874
State	Published - Feb 2022

ASJC Scopus subject areas

Epidemiology
Microbiology (medical)
Infectious Diseases

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title = "Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019",

abstract = "Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a nonenveloped, linear, single-stranded DNA virus belonging to the family Parvoviridae and is a World Organisation for Animal Health (OIE)-notifi able crustacean pathogen. During screening of Penaeus vannamei shrimp from 3 commercial shrimp facilities in the United States for a panel of OIE-listed (n = 7) and nonlisted (n = 2) crustacean diseases, shrimp from these facilities tested positive for IHHNV. Nucleotide sequences of PCR amplicons showed 99%-100% similarity to IHHNV isolates from Latin America and Asia. The whole genome of the isolates also showed high similarity to type 2 infectious forms of IHHNV. Phylogenetic analysis using capsid gene and whole-genome sequences demonstrated that the isolates clustered with an IHHNV isolate from Ecuador. The detection of an OIE-listed crustacean pathogen in the United States highlights the need for biosecurity protocols in hatcheries and grow-out ponds to mitigate losses.",

author = "Dhar, {Arun K.} and Roberto Cruz-Flores and Janet Warg and Killian, {Mary L.} and Andrew Orry and Jorge Ramos and Michelle Garfias and Gregory Lyons",

note = "Funding Information: Funding for this research was provided by the Aquaculture Pathology Laboratory Disease Diagnostic Fund. Partial funding was provided by the College of Agriculture & Life Sciences of The University of Arizona to A.K.D. Publisher Copyright: {\textcopyright} 2022 Centers for Disease Control and Prevention (CDC). All rights reserved.",

year = "2022",

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doi = "10.3201/eid2802.211874",

language = "English (US)",

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AU - Dhar, Arun K.

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AU - Warg, Janet

AU - Killian, Mary L.

AU - Orry, Andrew

AU - Ramos, Jorge

AU - Garfias, Michelle

AU - Lyons, Gregory

N1 - Funding Information: Funding for this research was provided by the Aquaculture Pathology Laboratory Disease Diagnostic Fund. Partial funding was provided by the College of Agriculture & Life Sciences of The University of Arizona to A.K.D. Publisher Copyright: © 2022 Centers for Disease Control and Prevention (CDC). All rights reserved.

PY - 2022/2

Y1 - 2022/2

N2 - Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a nonenveloped, linear, single-stranded DNA virus belonging to the family Parvoviridae and is a World Organisation for Animal Health (OIE)-notifi able crustacean pathogen. During screening of Penaeus vannamei shrimp from 3 commercial shrimp facilities in the United States for a panel of OIE-listed (n = 7) and nonlisted (n = 2) crustacean diseases, shrimp from these facilities tested positive for IHHNV. Nucleotide sequences of PCR amplicons showed 99%-100% similarity to IHHNV isolates from Latin America and Asia. The whole genome of the isolates also showed high similarity to type 2 infectious forms of IHHNV. Phylogenetic analysis using capsid gene and whole-genome sequences demonstrated that the isolates clustered with an IHHNV isolate from Ecuador. The detection of an OIE-listed crustacean pathogen in the United States highlights the need for biosecurity protocols in hatcheries and grow-out ponds to mitigate losses.

AB - Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is a nonenveloped, linear, single-stranded DNA virus belonging to the family Parvoviridae and is a World Organisation for Animal Health (OIE)-notifi able crustacean pathogen. During screening of Penaeus vannamei shrimp from 3 commercial shrimp facilities in the United States for a panel of OIE-listed (n = 7) and nonlisted (n = 2) crustacean diseases, shrimp from these facilities tested positive for IHHNV. Nucleotide sequences of PCR amplicons showed 99%-100% similarity to IHHNV isolates from Latin America and Asia. The whole genome of the isolates also showed high similarity to type 2 infectious forms of IHHNV. Phylogenetic analysis using capsid gene and whole-genome sequences demonstrated that the isolates clustered with an IHHNV isolate from Ecuador. The detection of an OIE-listed crustacean pathogen in the United States highlights the need for biosecurity protocols in hatcheries and grow-out ponds to mitigate losses.

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Genetic Relatedness of Infectious Hypodermal and Hematopoietic Necrosis Virus Isolates, United States, 2019

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