Genetic and phenotypic characterization of the blaCMY gene from escherichia coli and salmonella enterica isolated from food-producing animals, humans, the environment, and retail meat

Luke C. Heider, Armando E. Hoet, Thomas E. Wittum, Margaret L. Khaitsa, Brenda C. Love, Carla L. Huston, Paul S. Morley, Julie A. Funk, Wondwossen A. Gebreyes

Research output: Contribution to journalArticlepeer-review

24 Scopus citations

Abstract

The blaCMY-2 family of the ampC β-lactamase genes confer broad-spectrum resistance to β-lactam antimicrobials, including ceftriaxone and ceftiofur, as well as to β-lactamase inhibitors, such as clavulanic acid. Organisms with the blaCMY-2 phenotype have been recovered from the environment and from retail meat products, posing a potential public health risk. The objectives of this study were to sequence the blaCMY-2 gene from Escherichia coli and Salmonella enterica from multiple sources that had a reduced susceptibility to ceftriaxone and to determine the effect of observed mutations in the blaCMY-2 gene on the antimicrobial resistance phenotype (spectrum and minimum inhibitory concentration/susceptibility patterns) of the isolates. The blaCMY-2 genes from 52 bacterial isolates were sequenced for this study. Sixty-two percent (32/52) were E. coli and 38% (20/52) were S. enterica. Of the 32 E. coli isolates, 30 were found to carry a β-lactamase gene that was 100% homologous to blaCMY-2. One of the E. coli isolates was found to contain a gene that was 90% homologous to blaCMY-2. This isolate also had lower minimum inhibitory concentrations to tetracyclines, streptomycin, and the sulfonamide antimicrobials than are commonly expected for isolates containing the bla CMY-2. Of the 20 genes obtained from Salmonella isolates, 8 (40%) were found to be homologous to blaCMY-2, with no altered susceptibility phenotypes observed.

Original languageEnglish (US)
Pages (from-to)1235-1240
Number of pages6
JournalFoodborne Pathogens and Disease
Volume6
Issue number10
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Animal Science and Zoology

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