TY - JOUR
T1 - Generation of lipoxygenase products in the avascular tissues of the eye
AU - Williams, Richard N.
AU - Delamere, Nicholas A.
AU - Paterson, Christopher A.
N1 - Funding Information:
This work was supported by USPHS research grant number EYO0506 from the National Eye Institute and by the Biomedical Research Grant Program, Division of Research Resources, National Institutes of Health. The authors thank E. Paterson for editorial assistance.
PY - 1985/12
Y1 - 1985/12
N2 - We have examined the ability of the avascular cornea and lens to generate lipoxygenase products from arachidonic acid. The cornea and lens were obtained from rabbit and frog eyes, and their ability to metabolize exogenous [14C]-arachidonic acid determined in vitro. The cornea of rabbits and frogs formed lipoxygenase products from arachidonic acid. Both rabbit and frog corneas were found to generate considerable quantities of 12-HETE, although other lipoxygenase products were also produced. In contrast, no lipoxygenase products were detected following incubations of rabbit or frog lens with arachidonic acid. Thus, the cornea and lens, the two avascular tissues in the eye, exhibit marked differences in their ability to metabolize arachidonic acid via lipoxygenase enzymes.
AB - We have examined the ability of the avascular cornea and lens to generate lipoxygenase products from arachidonic acid. The cornea and lens were obtained from rabbit and frog eyes, and their ability to metabolize exogenous [14C]-arachidonic acid determined in vitro. The cornea of rabbits and frogs formed lipoxygenase products from arachidonic acid. Both rabbit and frog corneas were found to generate considerable quantities of 12-HETE, although other lipoxygenase products were also produced. In contrast, no lipoxygenase products were detected following incubations of rabbit or frog lens with arachidonic acid. Thus, the cornea and lens, the two avascular tissues in the eye, exhibit marked differences in their ability to metabolize arachidonic acid via lipoxygenase enzymes.
KW - arachidonic acid
KW - cornea
KW - lens
KW - lipoxygenase
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U2 - 10.1016/0014-4835(85)90181-2
DO - 10.1016/0014-4835(85)90181-2
M3 - Article
C2 - 3007188
AN - SCOPUS:0022324403
SN - 0014-4835
VL - 41
SP - 733
EP - 738
JO - Experimental eye research
JF - Experimental eye research
IS - 6
ER -