Abstract
Genomic and gene-specific DNA interstrand cross-links produced by nitrogen mustard (HN2) were measured in the human tumor cell line Colo320HSR. Following exposures that produced greater than or equal to 1 log cell kill, it was found that HN2-induced DNA interstrand cross-links were produced and processed in a heterogeneous fashion within the genome. Cross-links were detected in the amplified, overexpressed c-myc oncogene, whereas in the weakly expressed N-ras gene and the nontranscribed, high copy number alpha-satellite sequence (of chromosome 20), cross-links were not detected. The cross-links in the c-myc oncogene disappeared more rapidly than total genomic cross-links. These results suggest that HN2-induced DNA interstrand cross-links are produced and processed in the genome in a nonrandom fashion.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-223 |
| Number of pages | 7 |
| Journal | Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research |
| Volume | 3 |
| Issue number | 4 |
| State | Published - Apr 1992 |
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology