Abstract
Background. Studies were carried out to characterize several biochemical features of cultured AT-1 cells. Methods and Results. These cells were obtained from a transplantable atrial cardiomyocyte tumor lineage. Reverse transcriptase-polymerase chain reaction-based analyses demonstrated that the pattern of gene expression of cultured AT-1 cells was similar to that of adult atrial myocytes. AT-1 cells expressed atrial natriuretic factor (ANF), α-cardiac myosin heavy chain, α-cardiac actin, and connexin43. Radioimmunoassays verified that the cells synthesized, stored, and secreted ANF. Through size-exclusion, reversed-phase, and carboxymethyl-ion-exchange high-performance liquid chromatography, it was shown that cultured AT-1 cells stored ANF as pro-ANF (ANF-[1-126]), which was cosecretionally processed quantitatively to ANF-(1-98) and the bioactive 28-amino-acid ANF-(99-126). In addition, cultured AT-1 cells secreted ANF at almost a sixfold greater rate in response to endothelin-1, a potent secretagogue of ANF. KCl, metenkephalinamide, isoproterenol, phenylephrine, and 12-O-tetradecanoyl-phorbol-13-acetate also stimulated ANF release. Conclusions. These studies, in combination with previous findings, demonstrated that cultured AT-1 cells, while maintaining the ability to proliferate, have retained functional, biochemical, and ultrastructural features that are characteristic of adult atrial myocytes.
Original language | English (US) |
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Pages (from-to) | 1835-1841 |
Number of pages | 7 |
Journal | Circulation |
Volume | 85 |
Issue number | 5 |
DOIs | |
State | Published - May 1992 |
Externally published | Yes |
Keywords
- Cardiac myocyte hyperplasia
- Cell culture
- SV40 T antigen
- Transgenic mice
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
- Physiology (medical)