G-quadruplex DNA binding by a series of carbocyanine dyes

Sean M. Kerwin; Daekyu Sun; Jonathan T. Kern; Anupama Rangan; Pei Wang Thomas

doi:10.1016/S0960-894X(01)00490-5

G-quadruplex DNA binding by a series of carbocyanine dyes

Sean M. Kerwin
, Daekyu Sun
, Jonathan T. Kern
, Anupama Rangan
, Pei Wang Thomas

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.

Original language	English (US)
Pages (from-to)	2411-2414
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	11
Issue number	18
DOIs	https://doi.org/10.1016/S0960-894X(01)00490-5
State	Published - Sep 17 2001
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

Access to Document

10.1016/S0960-894X(01)00490-5

Cite this

@article{839853c8e0ab4981ad515694bae2ddd6,

title = "G-quadruplex DNA binding by a series of carbocyanine dyes",

abstract = "We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.",

author = "Kerwin, \{Sean M.\} and Daekyu Sun and Kern, \{Jonathan T.\} and Anupama Rangan and Thomas, \{Pei Wang\}",

note = "Funding Information: This work was supported by the National Institutes of Health (CA-67760) and the Robert Welch Foundation (F-1298).",

year = "2001",

month = sep,

day = "17",

doi = "10.1016/S0960-894X(01)00490-5",

language = "English (US)",

volume = "11",

pages = "2411--2414",

journal = "Bioorganic and Medicinal Chemistry Letters",

issn = "0960-894X",

publisher = "Elsevier Ltd",

number = "18",

}

TY - JOUR

T1 - G-quadruplex DNA binding by a series of carbocyanine dyes

AU - Kerwin, Sean M.

AU - Sun, Daekyu

AU - Kern, Jonathan T.

AU - Rangan, Anupama

AU - Thomas, Pei Wang

N1 - Funding Information: This work was supported by the National Institutes of Health (CA-67760) and the Robert Welch Foundation (F-1298).

PY - 2001/9/17

Y1 - 2001/9/17

N2 - We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.

AB - We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.

UR - https://www.scopus.com/pages/publications/0035903926

UR - https://www.scopus.com/pages/publications/0035903926#tab=citedBy

U2 - 10.1016/S0960-894X(01)00490-5

DO - 10.1016/S0960-894X(01)00490-5

M3 - Article

C2 - 11549435

AN - SCOPUS:0035903926

SN - 0960-894X

VL - 11

SP - 2411

EP - 2414

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 18

ER -

G-quadruplex DNA binding by a series of carbocyanine dyes

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this