G-quadruplex DNA binding by a series of carbocyanine dyes

Sean M. Kerwin; Daekyu Sun; Jonathan T. Kern; Anupama Rangan; Pei Wang Thomas

doi:10.1016/S0960-894X(01)00490-5

G-quadruplex DNA binding by a series of carbocyanine dyes

Sean M. Kerwin, Daekyu Sun, Jonathan T. Kern, Anupama Rangan, Pei Wang Thomas

Research output: Contribution to journal › Article › peer-review

47 Scopus citations

Abstract

We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.

Original language	English (US)
Pages (from-to)	2411-2414
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	11
Issue number	18
DOIs	https://doi.org/10.1016/S0960-894X(01)00490-5
State	Published - Sep 17 2001
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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title = "G-quadruplex DNA binding by a series of carbocyanine dyes",

abstract = "We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.",

author = "Kerwin, {Sean M.} and Daekyu Sun and Kern, {Jonathan T.} and Anupama Rangan and Thomas, {Pei Wang}",

note = "Funding Information: This work was supported by the National Institutes of Health (CA-67760) and the Robert Welch Foundation (F-1298).",

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AU - Kerwin, Sean M.

AU - Sun, Daekyu

AU - Kern, Jonathan T.

AU - Rangan, Anupama

AU - Thomas, Pei Wang

N1 - Funding Information: This work was supported by the National Institutes of Health (CA-67760) and the Robert Welch Foundation (F-1298).

PY - 2001/9/17

Y1 - 2001/9/17

N2 - We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.

AB - We have examined a number of carbocyanine dyes for their ability to bind intramolecular G-quadruplex DNA structures (G4′-DNA) using a Taq polymerase stop assay. Of the five dyes examined, only one, N,N'-diethylthiacarbocyanine iodide (DTC), was found to bind to G4′-DNA. DTC was also the only dye found to inhibit human telomerase at 50 μM concentration.

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G-quadruplex DNA binding by a series of carbocyanine dyes

Abstract

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