Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells

Nathan A. Groathouse; Robert A. Heinzen; Scott Boitano

doi:10.1128/IAI.71.12.7208-7210.2003

Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca²⁺ Transients in Ciliated Tracheal Epithelial Cells

Nathan A. Groathouse
, Robert A. Heinzen
, Scott Boitano

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca²⁺ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca²⁺ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca²⁺ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.

Original language	English (US)
Pages (from-to)	7208-7210
Number of pages	3
Journal	Infection and Immunity
Volume	71
Issue number	12
DOIs	https://doi.org/10.1128/IAI.71.12.7208-7210.2003
State	Published - Dec 2003

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/IAI.71.12.7208-7210.2003

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title = "Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells",

abstract = "To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.",

author = "Groathouse, \{Nathan A.\} and Heinzen, \{Robert A.\} and Scott Boitano",

year = "2003",

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doi = "10.1128/IAI.71.12.7208-7210.2003",

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journal = "Infection and Immunity",

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publisher = "American Society for Microbiology",

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T1 - Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells

AU - Groathouse, Nathan A.

AU - Heinzen, Robert A.

AU - Boitano, Scott

PY - 2003/12

Y1 - 2003/12

N2 - To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.

AB - To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.

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AN - SCOPUS:0345283032

SN - 0019-9567

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ER -

Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca²⁺ Transients in Ciliated Tracheal Epithelial Cells

Abstract

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