TY - JOUR
T1 - Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca2+ Transients in Ciliated Tracheal Epithelial Cells
AU - Groathouse, Nathan A.
AU - Heinzen, Robert A.
AU - Boitano, Scott
PY - 2003/12
Y1 - 2003/12
N2 - To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.
AB - To study initial Bordetella bronchiseptica-tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica-host cell interactions that can contribute to pathogenicity or to host cell defense.
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U2 - 10.1128/IAI.71.12.7208-7210.2003
DO - 10.1128/IAI.71.12.7208-7210.2003
M3 - Article
C2 - 14638818
AN - SCOPUS:0345283032
SN - 0019-9567
VL - 71
SP - 7208
EP - 7210
JO - Infection and Immunity
JF - Infection and Immunity
IS - 12
ER -