TY - JOUR
T1 - FTIR spectroscopic study of biogenic Mn-oxide formation by Pseudomonas putida GB-1
AU - Parikh, Sanjai J.
AU - Chorover, Jon
N1 - Funding Information:
We thank Dr. Bradley M. Tebo and Brian Clement for donation of GB-1 cells and providing information critical for cell growth and Mn-oxidation. We also thank Martha Conklin for useful discussions at early stages of this research and Hanna L. Gilbert for her assistance with SEM-EDS analysis. TEM-EDS assistance and analysis was provided by Sunkyung Choi and David Bentley. This research was supported by the National Science Foundation CRAEMS program (Grant CHE-0089156).
PY - 2005/7
Y1 - 2005/7
N2 - Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnOx, whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., "acid" birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnOx accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.
AB - Biomineralization in heterogeneous aqueous systems results from a complex association between pre-existing surfaces, bacterial cells, extracellular biomacromolecules, and neoformed precipitates. Fourier transform infrared (FTIR) spectroscopy was used in several complementary sample introduction modes (attenuated total reflectance [ATR], diffuse reflectance [DRIFT], and transmission) to investigate the processes of cell adhesion, biofilm growth, and biological Mn-oxidation by Pseudomonas putida strain GB-1. Distinct differences in the adhesive properties of GB-1 were observed upon Mn oxidation. No adhesion to the ZnSe crystal surface was observed for planktonic GB-1 cells coated with biogenic MnOx, whereas cell adhesion was extensive and a GB-1 biofilm was readily grown on ZnSe, CdTe, and Ge crystals prior to Mn-oxidation. IR peak intensity ratios reveal changes in biomolecular (carbohydrate, phosphate, and protein) composition during biologically catalyzed Mn-oxidation. In situ monitoring via ATR-FTIR of an active GB-1 biofilm and DRIFT data revealed an increase in extracellular protein (amide I and II) during Mn(II) oxidation, whereas transmission mode measurements suggest an overall increase in carbohydrate and phosphate moieties. The FTIR spectrum of biogenic Mn oxide comprises Mn-O stretching vibrations characteristic of various known Mn oxides (e.g., "acid" birnessite, romanechite, todorokite), but it is not identical to known synthetic solids, possibly because of solid-phase incorporation of biomolecular constituents. The results suggest that, when biogenic MnOx accumulates on the surfaces of planktonic cells, adhesion of the bacteria to other negatively charged surfaces is hindered via blocking of surficial proteins.
KW - Bacterial adhesion
KW - Biomineralization
KW - FTIR spectroscopy
KW - Mn oxidizing bacteria
KW - Mn(IV) oxides
KW - Pseudomonas putida
UR - http://www.scopus.com/inward/record.url?scp=21344452069&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21344452069&partnerID=8YFLogxK
U2 - 10.1080/01490450590947724
DO - 10.1080/01490450590947724
M3 - Article
AN - SCOPUS:21344452069
SN - 0149-0451
VL - 22
SP - 207
EP - 218
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 5
ER -