Frontiers in the measurement of cell and tissue pH

Robert J. Gillies, Ronald M. Lynch

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Investigation into the causes and consequences of aberrant pH regulation is entirely dependent on the ability to measure this parameter with specificity and sensitivity. Since their beginnings in the 1950s, techniques for measuring cell and tissue pH have undergone a number of significant advances. Following each of these advances, new investigators have been brought into the field, and new discoveries have been made. From microelectrode and dye distribution studies, measurement of pH underwent a revolution with the advent of pH-sensitive dyes that could be loaded into the cytosol. A further significant advance came from the measurement of cell and tissue pH in whole organisms by magnetic resonance spectroscopy (MRS). Frontiers in pH measurement exist in the development of pH-sensitive proteins and pH-sensitive MR contrast agents. These are predicted to bring even more people into this fascinating field, and generate more important discoveries.

Original languageEnglish (US)
Pages (from-to)7-19
Number of pages13
JournalNovartis Foundation Symposium
StatePublished - 2001

ASJC Scopus subject areas

  • Medicine(all)


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