Frequency dependent 2H N.M.R. relaxation rates of small unilamellar phospholipid vesicles

Michael F. Brown; Amir Salmon; Ulf Henriksson; Olle Söderman

doi:10.1080/00268979000100271

Frequency dependent ²H N.M.R. relaxation rates of small unilamellar phospholipid vesicles

Michael F. Brown, Amir Salmon, Ulf Henriksson, Olle Söderman

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Deuterium (²H) N.M.R. spectroscopy has been used to investigate experimentally the molecular dynamics of phospholipid vesicles. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was deuterated specifically in both acyl chains, and aqueous suspensions of vesicles were studied above the main order-disorder transition temperature. ²H spin-lattice relaxation rates (R₁)were measured at nine different magnetic field strengths, corresponding to Larmor frequencies (v₀ = ω₀/2Π) spanning 2.5 MHz to 61.4 MHz. Of three models considered, a model in which R₁ ∝ω₀^-1/2 best described the ²H N.M.R. results, whereas fits to R₁∝ ω₀^-1 or to a lorentzian plus a constant dispersion were less satisfactory. Thus a broad distribution of motions exists, which must be explained by any model for the N.M.R. relaxation of liquid-crystalline phospholipids.

Original language	English (US)
Pages (from-to)	379-383
Number of pages	5
Journal	Molecular Physics
Volume	69
Issue number	2
DOIs	https://doi.org/10.1080/00268979000100271
State	Published - Feb 10 1990

ASJC Scopus subject areas

Biophysics
Molecular Biology
Condensed Matter Physics
Physical and Theoretical Chemistry

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title = "Frequency dependent 2H N.M.R. relaxation rates of small unilamellar phospholipid vesicles",

abstract = "Deuterium (2H) N.M.R. spectroscopy has been used to investigate experimentally the molecular dynamics of phospholipid vesicles. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was deuterated specifically in both acyl chains, and aqueous suspensions of vesicles were studied above the main order-disorder transition temperature. 2H spin-lattice relaxation rates (R1)were measured at nine different magnetic field strengths, corresponding to Larmor frequencies (v0 = ω0/2Π) spanning 2.5 MHz to 61.4 MHz. Of three models considered, a model in which R1 ∝ω0-1/2 best described the 2H N.M.R. results, whereas fits to R1∝ ω0-1 or to a lorentzian plus a constant dispersion were less satisfactory. Thus a broad distribution of motions exists, which must be explained by any model for the N.M.R. relaxation of liquid-crystalline phospholipids.",

author = "Brown, {Michael F.} and Amir Salmon and Ulf Henriksson and Olle S{\"o}derman",

note = "Funding Information: We thank Peter Stilbs, Stockholm, and Per Linse, Lund, for providing the computer programs and Hfikan Wennerstrfm, Lund, for helpful discussions. Work sponsored by grants GM41413, EY03754, and RR03529 from the U.S. National Institutes of Health, and by the Swedish Natural Sciences Research Council.",

year = "1990",

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doi = "10.1080/00268979000100271",

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AU - Brown, Michael F.

AU - Salmon, Amir

AU - Henriksson, Ulf

AU - Söderman, Olle

N1 - Funding Information: We thank Peter Stilbs, Stockholm, and Per Linse, Lund, for providing the computer programs and Hfikan Wennerstrfm, Lund, for helpful discussions. Work sponsored by grants GM41413, EY03754, and RR03529 from the U.S. National Institutes of Health, and by the Swedish Natural Sciences Research Council.

PY - 1990/2/10

Y1 - 1990/2/10

N2 - Deuterium (2H) N.M.R. spectroscopy has been used to investigate experimentally the molecular dynamics of phospholipid vesicles. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was deuterated specifically in both acyl chains, and aqueous suspensions of vesicles were studied above the main order-disorder transition temperature. 2H spin-lattice relaxation rates (R1)were measured at nine different magnetic field strengths, corresponding to Larmor frequencies (v0 = ω0/2Π) spanning 2.5 MHz to 61.4 MHz. Of three models considered, a model in which R1 ∝ω0-1/2 best described the 2H N.M.R. results, whereas fits to R1∝ ω0-1 or to a lorentzian plus a constant dispersion were less satisfactory. Thus a broad distribution of motions exists, which must be explained by any model for the N.M.R. relaxation of liquid-crystalline phospholipids.

AB - Deuterium (2H) N.M.R. spectroscopy has been used to investigate experimentally the molecular dynamics of phospholipid vesicles. 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) was deuterated specifically in both acyl chains, and aqueous suspensions of vesicles were studied above the main order-disorder transition temperature. 2H spin-lattice relaxation rates (R1)were measured at nine different magnetic field strengths, corresponding to Larmor frequencies (v0 = ω0/2Π) spanning 2.5 MHz to 61.4 MHz. Of three models considered, a model in which R1 ∝ω0-1/2 best described the 2H N.M.R. results, whereas fits to R1∝ ω0-1 or to a lorentzian plus a constant dispersion were less satisfactory. Thus a broad distribution of motions exists, which must be explained by any model for the N.M.R. relaxation of liquid-crystalline phospholipids.

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Frequency dependent ²H N.M.R. relaxation rates of small unilamellar phospholipid vesicles

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