Freeze fracture of monolayer cultures

B. U. Pauli, R. S. Weinstein, L. W. Soble, J. Alroy

Research output: Contribution to journalArticlepeer-review

79 Scopus citations

Abstract

This paper describes a simple method for the freeze fracturing of cells in monolayers or multilayer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze fracturing.

Original languageEnglish (US)
Pages (from-to)763-769
Number of pages7
JournalJournal of Cell Biology
Volume72
Issue number3
DOIs
StatePublished - 1977

ASJC Scopus subject areas

  • Cell Biology

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