Folate functionalized PLGA nanoparticles loaded with plasmid pVAX1-NH36: Mathematical analysis of release

Cindy Alejandra Gutiérrez-Valenzuela; Patricia Guerrero-Germán; Armando Tejeda-Mansir; Reynaldo Esquivel; Roberto Guzmán-Z; Armando Lucero-Acuña

doi:10.3390/app6120364

Folate functionalized PLGA nanoparticles loaded with plasmid pVAX1-NH36: Mathematical analysis of release

Cindy Alejandra Gutiérrez-Valenzuela, Patricia Guerrero-Germán, Armando Tejeda-Mansir, Reynaldo Esquivel, Roberto Guzmán-Z, Armando Lucero-Acuña

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-coglycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below -24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.

Original language	English (US)
Article number	364
Journal	Applied Sciences (Switzerland)
Volume	6
Issue number	12
DOIs	https://doi.org/10.3390/app6120364
State	Published - 2016

Keywords

Copolymer synthesis
Drug delivery
Folate nanoparticles
Leishmaniasis
Mathematical analysis
PVAX1-NH36

ASJC Scopus subject areas

General Materials Science
Instrumentation
General Engineering
Process Chemistry and Technology
Computer Science Applications
Fluid Flow and Transfer Processes

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10.3390/app6120364

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title = "Folate functionalized PLGA nanoparticles loaded with plasmid pVAX1-NH36: Mathematical analysis of release",

abstract = "Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-coglycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below -24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.",

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author = "Guti{\'e}rrez-Valenzuela, {Cindy Alejandra} and Patricia Guerrero-Germ{\'a}n and Armando Tejeda-Mansir and Reynaldo Esquivel and Roberto Guzm{\'a}n-Z and Armando Lucero-Acu{\~n}a",

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doi = "10.3390/app6120364",

language = "English (US)",

volume = "6",

journal = "Applied Sciences (Switzerland)",

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T1 - Folate functionalized PLGA nanoparticles loaded with plasmid pVAX1-NH36

T2 - Mathematical analysis of release

AU - Gutiérrez-Valenzuela, Cindy Alejandra

AU - Guerrero-Germán, Patricia

AU - Tejeda-Mansir, Armando

AU - Esquivel, Reynaldo

AU - Guzmán-Z, Roberto

AU - Lucero-Acuña, Armando

PY - 2016

Y1 - 2016

N2 - Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-coglycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below -24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.

AB - Plasmid DNA (pVAX1-NH36) was encapsulated in nanoparticles of poly-dl-lactic-coglycolic (PLGA) functionalized with polyethylene glycol (PEG) and folic acid (PLGA-PEG-FA) without losing integrity. PLGA-PEG-FA nanoparticles loaded with pVAX1-NH36 (pDNA-NPs) were prepared by using a double emulsification-solvent evaporation technique. PLGA-PEG-FA synthesis was verified by FT-IR and spectrophotometry methods. pVAX1-NH36 was replicated in Escherichia coli (E. coli) cell cultures. Atomic force microscopy (AFM) analysis confirmed pDNA-NPs size with an average diameter of 177-229 nm, depending on pVAX1-NH36 loading and zeta potentials were below -24 mV for all preparations. In vitro release studies confirmed a multiphase release profile for the duration of more than 30-days. Plasmid release kinetics were analyzed with a release model that considered simultaneous contributions of initial burst and degradation-relaxation of nanoparticles. Fitting of release model against experimental data presented excellent correlation. This mathematical analysis presents a novel approach to describe and predict the release of plasmid DNA from biodegradable nanoparticles.

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KW - Leishmaniasis

KW - Mathematical analysis

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Folate functionalized PLGA nanoparticles loaded with plasmid pVAX1-NH36: Mathematical analysis of release

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