TY - GEN
T1 - Fluorescent and scattering contrast agents in a mouse model of colorectal cancer
AU - Winkler, Amy M.
AU - Rice, Photini F.S.
AU - Troutman, Timothy S.
AU - Backer, Marina V.
AU - Backer, Joseph M.
AU - Drezek, Rebekah A.
AU - Romanowski, Marek
AU - Barton, Jennifer K.
PY - 2008
Y1 - 2008
N2 - In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..
AB - In previous work we have demonstrated the utility of laser-induced fluorescence (LIF) and optical coherence tomography (OCT) to identify adenoma in mouse models of colorectal cancer with high sensitivity and specificity. However, improved sensitivity to early disease, as well as the ability to distinguish confounders (e.g. fecal contamination, natural variations in mucosal thickness), is desired. In this study, we investigated the signal enhancement of fluorescent and scattering contrast agents in the colons of AOM-treated mice. The fluorescent tracer scVEGF/Cy, targeted to receptors for vascular endothelial growth factor, was visualized on a dual modality OCT/LIF endoscopic system with 1300-nm center wavelength OCT source and 635-nm LIF excitation. Scattering agents were tested with an 890-nm center wavelength endoscopic OCT system. Agents included nanoshells, 120-nm in diameter, and nanorods, 20-nm in diameter by 80-nm in length. Following imaging, colons were excised. Tissue treated with fluorophore was imaged on an epifluorescence microscope. Histological sections were obtained and stained with H&E and silver enhancer to verify disease and identify regions of gold uptake, respectively. Non-specific signal enhancement was observed with the scattering contrast agents. Specificity for adenoma was seen with the scVEGF/Cy dye..
KW - Colon cancer
KW - Laser-induced fluorescence
KW - Optical coherence tomography
UR - http://www.scopus.com/inward/record.url?scp=42149125612&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=42149125612&partnerID=8YFLogxK
U2 - 10.1117/12.764043
DO - 10.1117/12.764043
M3 - Conference contribution
AN - SCOPUS:42149125612
SN - 9780819470263
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Endoscopic Microscopy III
T2 - Endoscopic Microscopy III
Y2 - 20 January 2008 through 21 January 2008
ER -