Fluorescent and Lanthanide Labeling for Ligand Screens, Assays, and Imaging

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The use of fluorescent (or luminescent) and metal contrast agents in high-throughput screens, in vitro assays, and molecular imaging procedures has rapidly expanded in recent years. Here we describe the development and utility of high-affinity ligands for cancer theranostics and other in vitro screening ­studies. In this context, we also illustrate the syntheses and use of heteromultivalent ligands as targeted imaging agents.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages89-126
Number of pages38
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume716
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cy5
  • DELFIA
  • DOTA
  • DTPA
  • Dual-modal agents
  • Heteromultivalent ligands,Lanthaligands
  • PARACEST MRI
  • Solid-phase synthesis
  • Targeted imaging agents

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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