Fluorescent and Lanthanide Labeling for Ligand Screens, Assays, and Imaging

Jatinder S. Josan, Channa R. De Silva, Byunghee Yoo, Ronald M. Lynch, Mark D. Pagel, Josef Vagner, Victor J. Hruby

Research output: Chapter in Book/Report/Conference proceedingChapter

20 Scopus citations

Abstract

The use of fluorescent (or luminescent) and metal contrast agents in high-throughput screens, in vitro assays, and molecular imaging procedures has rapidly expanded in recent years. Here we describe the development and utility of high-affinity ligands for cancer theranostics and other in vitro screening ­studies. In this context, we also illustrate the syntheses and use of heteromultivalent ligands as targeted imaging agents.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages89-126
Number of pages38
DOIs
StatePublished - 2011

Publication series

NameMethods in Molecular Biology
Volume716
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cy5
  • DELFIA
  • DOTA
  • DTPA
  • Dual-modal agents
  • Heteromultivalent ligands,Lanthaligands
  • PARACEST MRI
  • Solid-phase synthesis
  • Targeted imaging agents

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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