TY - JOUR
T1 - Fluorescence polarization immunoassay, IV Determination of phenytoin and phenobarbital in human serum and plasma
AU - Lu-Steffes, M.
AU - Pittluck, G. W.
AU - Jolley, M. E.
AU - Panas, H. N.
AU - Olive, D. L.
AU - Wang, C. H.
AU - Nystrom, D. D.
AU - Keegan, C. L.
AU - Davis, T. P.
AU - Stroupe, S. D.
PY - 1982
Y1 - 1982
N2 - Fluorescence polarization immunoassays for phenytoin and phenobarbital in human serum or plasma are described and shown to be clinically useful. No sample pretreatment, extraction, or phase separation is required. A determination can be made with less than 20 μL of sample in 15 min, with incubation at ambient temperature. Abnormal concentrations of protein, lipid, hemoglobin, or bilirubin do not interfere. In addition, drug metabolites and commonly co-administered drugs do not affect the assay results at clinically significant concentrations. Analytical recoveries of each of the two anticonvulsant drugs from serum averaged 101%. Between-assay CVs were <6.5%, with sensitivities of 0.5 mg/L. Comparison of this method with 'high-performance' liquid chromatography, homogeneous enzyme immunoassay, and radioimmunoassay for phenytoin determinations yielded correlations of 0.99, 0.98, and 0.99, respectively. Similar comparison studies for phenobarbital yielded correlation coefficients of 0.99, 0.98, and 0.99, respectively.
AB - Fluorescence polarization immunoassays for phenytoin and phenobarbital in human serum or plasma are described and shown to be clinically useful. No sample pretreatment, extraction, or phase separation is required. A determination can be made with less than 20 μL of sample in 15 min, with incubation at ambient temperature. Abnormal concentrations of protein, lipid, hemoglobin, or bilirubin do not interfere. In addition, drug metabolites and commonly co-administered drugs do not affect the assay results at clinically significant concentrations. Analytical recoveries of each of the two anticonvulsant drugs from serum averaged 101%. Between-assay CVs were <6.5%, with sensitivities of 0.5 mg/L. Comparison of this method with 'high-performance' liquid chromatography, homogeneous enzyme immunoassay, and radioimmunoassay for phenytoin determinations yielded correlations of 0.99, 0.98, and 0.99, respectively. Similar comparison studies for phenobarbital yielded correlation coefficients of 0.99, 0.98, and 0.99, respectively.
UR - http://www.scopus.com/inward/record.url?scp=0020461593&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020461593&partnerID=8YFLogxK
U2 - 10.1093/clinchem/28.11.2278
DO - 10.1093/clinchem/28.11.2278
M3 - Article
C2 - 6751601
AN - SCOPUS:0020461593
SN - 0375-9474
VL - 28
SP - 2278
EP - 2282
JO - Unknown Journal
JF - Unknown Journal
IS - 11
ER -