Abstract
Bleach rate imaging on a (cooled) CCD can be easily achieved using a confocal microscope with bilateral scanning and detection coupled to a workstation; it is as easy as acquiring regular fluorescence images. Several analysis and display methods for bleach rate imaging are presented such as the bleach map (and its inverse) and a matrix‐based decomposition method for multi‐labelled specimens based on the bleach rate differences between the dyes used. With these tools, bleach‐rate‐based imaging can become a viable alternative to multiple labelling techniques for component identification in fluorescent specimens. 1994 Blackwell Science Ltd
| Original language | English (US) |
|---|---|
| Pages (from-to) | 154-161 |
| Number of pages | 8 |
| Journal | Journal of Microscopy |
| Volume | 175 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 1994 |
| Externally published | Yes |
Keywords
- CCD
- Fluorescence microscopy
- bilateral
- bleach map
- bleach rate
- confocal
- histomap
- photobleaching
- photodegradation
- ratio‐imaging
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Histology
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