@article{86a6a79fa95046e69a7414b42ca8f452,
title = "Fluorescence activated cell sorting—A selective tool for plant cell isolation and analysis",
abstract = "Instrumentation for flow cytometry and sorting is designed around the assumption that samples are single-cell suspensions. However, with few exceptions, higher plants comprise complex multicellular tissues and organs, in which the individual cells are held together by shared cell walls. Single-cell suspensions can be obtained through digestion of the cells walls and release of the so-called protoplasts (plants without their cell wall). Here we describe best practices for protoplast preparation, and for analysis through flow cytometry and cell sorting. Finally, the numerous downstream applications involving sorted protoplasts are discussed.",
keywords = "autofluorescence, best practices, plant flow cytometry and sorting, protoplasts, viability and integrity",
author = "Ioanna Antoniadi and Vladim{\'i}r Skalick{\'y} and Guiling Sun and Wen Ma and Galbraith, {David W.} and Ond{\v r}ej Nov{\'a}k and Karin Ljung",
note = "Funding Information: D.W.G. acknowledges on‐going support from the USDA through the University of Arizona College of Agriculture and Life Sciences, and with G.S. and W.M., from the Chinese National Key Research and Development Program, the National Natural Science Foundation of China, the Program for Innovative Research Teams in Science and Technology at a University of Henan Province, and the 111 Project of China. This work was also supported by grants from The Czech Foundation Agency (GA17‐21581Y), the Internal Grant Agency of Palack{\'y} University (IGA_PrF_2021_011), and the Ministry of Education, Youth and Sport of the Czech Republic via the ERDF project “Plants as a tool for sustainable global development” (CZ.02.1.01/0.0/0.0/16_019/0000827). V.S. was partially supported by the Endowment fund of Palack{\'y} University in Olomouc. The authors also acknowledge the European Molecular Biology Organization (EMBO ASTF 297‐2013) (I.A.) and Plant Fellows (the International Post doc Fellowship Program in Plant Sciences, 267423) (I.A.; K.L.), the Swedish Research Council (VR), the VINNOVA (Vinnova), the Knut och Alice Wallenbergs Stiftelse (KAW) and Kempestiftelserna (K.L.). Funding Information: Chinese National Key Research and Development Program, the National Natural Science Foundation of China, the Program for Innovative Research Teams in Science and Technology at a University of Henan Province, and the 111 Project of China; Czech Foundation Agency, Grant/Award Number: GA17‐21581Y; Endowment fund of Palack{\'y} University in Olomouc; European Molecular Biology Organization, Grant/Award Number: EMBO ASTF 297‐2013; Internal Grant Agency of Palack{\'y} University, Grant/Award Number: IGA_PrF_2021_011; Kempestiftelserna; Ministry of Education, Youth and Sport of the Czech Republic via the ERDF project “Plants as a tool for sustainable global development”, Grant/Award Number: CZ.02.1.01/0.0/0.0/16_019/0000827; Plant Fellows (the International Post doc Fellowship Programme in Plant Sciences, Grant/Award Number: 267423; the Knut and Alice Wallenberg Foundation (KAW); the Swedish Governmental Agency for Innovation Systems (Vinnova); the Swedish Research Council; University of Arizona College of Agriculture and Life Sciences Funding information Publisher Copyright: {\textcopyright} 2021 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.",
year = "2022",
month = sep,
doi = "10.1002/cyto.a.24461",
language = "English (US)",
volume = "101",
pages = "725--736",
journal = "Cytometry. Part A : the journal of the International Society for Analytical Cytology",
issn = "1552-4922",
publisher = "Wiley-Liss Inc.",
number = "9",
}