Flow injection-chemiluminescent assay for the determination of superoxide dismutase activity

H. Y. Choi, J. H. Song, Y. S. Park, G. Lord, D. K. Park

Research output: Contribution to journalArticlepeer-review

Abstract

Superoxide dismutase (SOD) activity was measured using a flow injection-chemiluminescent assay (FI-CLA) based on xanthine-xanthine oxidase dependent superoxide (O2·-) formation. The mobile phase consists of 50 mM potassium phosphate buffer (pH 7.4) containing lucigenin (5 μM) and xanthine (0.3 mM). Under optimum conditions, bovine albumin did not affect chemiluminescence at concentrations of 1-1000 μg mL-1 and KCN inhibited 100% of the Cu, Zn-SOD activity using 5 μL of a 0.23 mM concentration. The analysis of one sample was done in less than 30 s with a relative standard deviation of ±3.1%. SOD activity in the biological samples was correlated to the amount of exogenously applied SOD. The FI-CLA method reported here appears to be one of the faster and more useful tools used to assay SOD activity.

Original languageEnglish (US)
Pages (from-to)337-341
Number of pages5
JournalCanadian Journal of Chemistry
Volume79
Issue number3
DOIs
StatePublished - 2001
Externally publishedYes

Keywords

  • Flow injection-chemiluminescent assay
  • Lucigenin
  • Superoxide
  • Superoxide dismutase
  • Xanthine oxidase

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Organic Chemistry

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