Fitness level impacts salivary antimicrobial protein responses to a single bout of cycling exercise

Hawley Kunz, Nicolette C. Bishop, Guillaume Spielmann, Mira Pistillo, Justin Reed, Teja Ograjsek, Yoonjung Park, Satish K. Mehta, Duane L. Pierson, Richard J. Simpson

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

Purpose: Salivary antimicrobial proteins (sAMPs) protect the upper respiratory tract (URTI) from invading microorganisms and have been linked with URTI infection risk in athletes. While high training volume is associated with increased URTI risk, it is not known if fitness affects the sAMP response to acute exercise. This study compared the sAMP responses to various exercising workloads of highly fit experienced cyclists with those who were less fit. Methods: Seventeen experienced cyclists (nine highly fit; eight less fit) completed three 30-min exercise trials at workloads corresponding to −5, +5 and +15 % of the individual blood lactate threshold. Saliva samples were collected pre- and post-exercise to determine the concentration and secretion of α-amylase, human neutrophil proteins 1–3 (HNP1–3) lactoferrin, LL-37, lysozyme, and salivary SIgA. Results: The concentration and/or secretion of all sAMPs increased post-exercise, but only α-amylase was sensitive to exercise workload. Highly fit cyclists had lower baseline concentrations of α-amylase, HNP1–3, and lactoferrin, although secretion rates did not differ between the groups. Highly fit cyclists did, however, exhibit greater post-exercise increases in the concentration and/or secretion of a majority of measured sAMPs (percentage difference between highly fit and less fit in parentheses), including α-amylase concentration (+107 %) and secretion (+148 %), HNP1–3 concentration (+97 %) and secretion (+158 %), salivary SIgA concentration (+181 %), lactoferrin secretion (+209 %) and LL-37 secretion (+138 %). Conclusion: We show for the first time that fitness level is a major determinant of exercise-induced changes in sAMPs. This might be due to training-induced alterations in parasympathetic and sympathetic nervous system activation.

Original languageEnglish (US)
Pages (from-to)1015-1027
Number of pages13
JournalEuropean Journal of Applied Physiology
Volume115
Issue number5
DOIs
StatePublished - May 1 2015
Externally publishedYes

Keywords

  • Exercise intensity
  • Exercise training
  • Fitness
  • Innate mucosal immunity
  • Salivary antimicrobial proteins

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Public Health, Environmental and Occupational Health
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Fitness level impacts salivary antimicrobial protein responses to a single bout of cycling exercise'. Together they form a unique fingerprint.

Cite this