Finger-stick blood sampling Methodology for the determination of exercise-induced lymphocyte apoptosis

James Navalta, Brian McFarlin, Richard Simpson, Elizabeth Fedor, Holly Kell, Scott Lyons, Scott Arnett, Mark Schafer

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Exercise is a physiological stimulus capable of inducing apoptosis in immune cells. To date, various limitations have been identified with the measurement of this phenomenon, particularly relating to the amount of time required to isolate and treat a blood sample prior to the assessment of cell death. Because of this, it is difficult to determine whether reported increases in immune cell apoptosis can be contributed to the actual effect of exercise on the system, or are a reflection of the time and processing necessary to eventually obtain this measurement. In this article we demonstrate a rapid and minimally invasive procedure for the analysis of exercise-induced lymphocyte apoptosis. Unlike other techniques, whole blood is added to an antibody panel immediately upon obtaining a sample. Following the incubation period, red blood cells are lysed and samples are ready to be analyzed. The use of a finger-stick sampling procedure reduces the volume of blood required, and minimizes the discomfort to subjects.

Original languageEnglish (US)
Article numbere2595
JournalJournal of Visualized Experiments
Issue number48
StatePublished - Feb 2011
Externally publishedYes


  • Immunology
  • Issue 48
  • Leukocyte phenotyping
  • Muscular activity
  • Programmed cell death
  • Technique development

ASJC Scopus subject areas

  • General Neuroscience
  • General Chemical Engineering
  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology


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