Abstract
We report the application of fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy. This novel, to our knowledge, label-free imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth-harmonic and five-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing.
Original language | English (US) |
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Pages (from-to) | 3931-3934 |
Number of pages | 4 |
Journal | Optics letters |
Volume | 50 |
Issue number | 12 |
DOIs | |
State | Published - Jun 15 2025 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics