Fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy

Joshua H. Magnus, Lam T. Nguyen, Elana G. Alevy, Travis W. Sawyer, Samuel D. Crossley, Khanh Kieu

Research output: Contribution to journalArticlepeer-review

Abstract

We report the application of fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy. This novel, to our knowledge, label-free imaging modality has been characterized with spectral and power dependence measurements. The advantages of fifth-harmonic and five-photon excitation fluorescence imaging are higher resolution and use of longer wavelength excitation sources, which may allow deeper penetration depth while still exciting near-UV to blue fluorophores. This imaging modality may find interesting applications in biological research, materials characterization, geologic studies, and semiconductor manufacturing.

Original languageEnglish (US)
Pages (from-to)3931-3934
Number of pages4
JournalOptics letters
Volume50
Issue number12
DOIs
StatePublished - Jun 15 2025

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Fingerprint

Dive into the research topics of 'Fifth-harmonic and five-photon excitation fluorescence multiphoton microscopy'. Together they form a unique fingerprint.

Cite this