Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

Ning Sun, Nicholas J. Panetta, Deepak M. Gupta, Kitchener D. Wilson, Andrew Lee, Fangjun Jia, Shijun Hu, Athena M. Cherry, Robert C. Robbins, Michael T. Longaker, Joseph C. Wu

Research output: Contribution to journalArticlepeer-review

439 Scopus citations

Abstract

Ectopic expression of transcription factors can reprogram somatic cells to a pluripotent state. However, most of the studies used skin fibroblasts as the starting population for reprogramming, which usually take weeks for expansion from a single biopsy. We show here that induced pluripotent stem (iPS) cells can be generated from adult human adipose stem cells (hASCs) freshly isolated from patients. Furthermore, iPS cells can be readily derived from adult hASCs in a feeder-free condition, thereby eliminating potential variability caused by using feeder cells. hASCs can be safely and readily isolated from adult humans in large quantities without extended time for expansion, are easy to maintain in culture, and therefore represent an ideal autologous source of cells for generating individual-specific iPS cells.

Original languageEnglish (US)
Pages (from-to)15720-15725
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume106
Issue number37
DOIs
StatePublished - Sep 15 2009
Externally publishedYes

Keywords

  • Differentiation
  • Pluripotency
  • Reprogramming

ASJC Scopus subject areas

  • General

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