Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization

Eric Chiles; Yujue Wang; Katarzyna M. Kalemba; Hyokjoon Kwon; Fredric E. Wondisford; Xiaoyang Su

doi:10.1016/j.ab.2019.03.020

Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization

Eric Chiles
, Yujue Wang
, Katarzyna M. Kalemba
, Hyokjoon Kwon
, Fredric E. Wondisford
, Xiaoyang Su

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. This derivatization protocol can be used to measure the concentrations of glucose production in a plethora of sample types for metabolic analysis and is compatible with the general metabolomics workflow. This novel approach allows us to quantitatively study glucose and glycerol metabolism using stable isotope tracers in vivo.

Original language	English (US)
Pages (from-to)	40-43
Number of pages	4
Journal	Analytical Biochemistry
Volume	575
DOIs	https://doi.org/10.1016/j.ab.2019.03.020
State	Published - Jun 15 2019
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.ab.2019.03.020

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title = "Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization",

abstract = "Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. This derivatization protocol can be used to measure the concentrations of glucose production in a plethora of sample types for metabolic analysis and is compatible with the general metabolomics workflow. This novel approach allows us to quantitatively study glucose and glycerol metabolism using stable isotope tracers in vivo.",

author = "Eric Chiles and Yujue Wang and Kalemba, \{Katarzyna M.\} and Hyokjoon Kwon and Wondisford, \{Fredric E.\} and Xiaoyang Su",

note = "Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",

year = "2019",

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doi = "10.1016/j.ab.2019.03.020",

language = "English (US)",

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journal = "Analytical Biochemistry",

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T1 - Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization

AU - Chiles, Eric

AU - Wang, Yujue

AU - Kalemba, Katarzyna M.

AU - Kwon, Hyokjoon

AU - Wondisford, Fredric E.

AU - Su, Xiaoyang

PY - 2019/6/15

Y1 - 2019/6/15

N2 - Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. This derivatization protocol can be used to measure the concentrations of glucose production in a plethora of sample types for metabolic analysis and is compatible with the general metabolomics workflow. This novel approach allows us to quantitatively study glucose and glycerol metabolism using stable isotope tracers in vivo.

AB - Glucose and glycerol are important circulating metabolites. Due to poor ionization and/or ion suppression, the liquid chromatography-mass spectrometry (LC-MS) detection of glucose and glycerol presents challenges. Here, we propose an efficient LC-MS method of quantitative glucose and glycerol detection via enzymatic derivatization to glucose-6-phosphate and sn-glycerol-3-phosphate, respectively. This derivatization protocol can be used to measure the concentrations of glucose production in a plethora of sample types for metabolic analysis and is compatible with the general metabolomics workflow. This novel approach allows us to quantitatively study glucose and glycerol metabolism using stable isotope tracers in vivo.

UR - https://www.scopus.com/pages/publications/85063762957

UR - https://www.scopus.com/pages/publications/85063762957#tab=citedBy

U2 - 10.1016/j.ab.2019.03.020

DO - 10.1016/j.ab.2019.03.020

M3 - Article

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AN - SCOPUS:85063762957

SN - 0003-2697

VL - 575

SP - 40

EP - 43

JO - Analytical Biochemistry

JF - Analytical Biochemistry

ER -

Fast LC-MS quantitation of glucose and glycerol via enzymatic derivatization

Abstract

ASJC Scopus subject areas

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