Fast Hadamard transform capillary electrophoresis for on-line, time-resolved chemical monitoring

Kevin L. Braun, Suminda Hapuarachchi, Facundo M. Fernandez, Craig A. Aspinwall

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


We report a new approach for collecting and deconvoluting the data in Hadamard transform capillary electrophoresis, referred to as fast Hadamard transform capillary electrophoresis (fHTCE). Using fHTCE, total analysis times can be reduced by up to 48% per multiplexed separation compared to conventional Hadamard transform capillary electrophoresis (cHTCE) while providing comparable signal-to-noise ratio enhancements. In fHT-CE, the sample is injected following a pseudorandom pulsing sequence derived from the first row of a simplex matrix (S-matrix) in contrast to cHTCE, which utilizes a sequence of twice the length. In addition to the temporal savings provided by fHTCE, a 50% reduction in sample consumption is also realized due to the decreased number of sample injections. We have applied fHTCE to the analysis of mixtures of neurotransmitters and related compounds to yield improved signal-to-noise ratios with a total analysis time under 10 s. In addition, we demonstrate the capability of fHTCE to perform time-resolved monitoring of changes in the concentration of model neurochemical compounds.

Original languageEnglish (US)
Pages (from-to)1628-1635
Number of pages8
JournalAnalytical Chemistry
Issue number5
StatePublished - Mar 1 2006

ASJC Scopus subject areas

  • Analytical Chemistry


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