Abstract
Activation of the Fas receptor (Fas; CD95) on eosinophils induces apoptosis and may play a role in controlling eosinophil number in blood and tissue. To determine whether Fas expression on eosinophils is different between asthmatics and nonasthmatics, eosinophils were isolated from the peripheral blood using a three-step procedure of dextran sedimentation, ficoll hypaque separation and negative selection with anti-CD16 monoclonal antibody bound to magnetic beads. Fas expression was quantified by flow cytometry. Fas expression and the percentage of eosinophils expressing Fas were not different between asthmatics (n=9) and non-asthmatics (n=14).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 93-96 |
| Number of pages | 4 |
| Journal | Research Communications in Molecular Pathology and Pharmacology |
| Volume | 102 |
| Issue number | 1 |
| State | Published - 1998 |
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology