Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum

Denis V. Yashin; Elena A. Dukhanina; Olga D. Kabanova; Elena A. Romanova; Tamara I. Lukyanova; Alexsander G. Tonevitskii; Alexey A. Belogurov; Deborah A. Raynes; Anton A. Sheludchenkov; Nikolai V. Gnuchev; Vince Guerriero; Georgii P. Georgiev; Lidia P. Sashchenko

doi:10.1016/j.biochi.2011.10.007

Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum

Denis V. Yashin, Elena A. Dukhanina, Olga D. Kabanova, Elena A. Romanova, Tamara I. Lukyanova, Alexsander G. Tonevitskii, Alexey A. Belogurov, Deborah A. Raynes, Anton A. Sheludchenkov, Nikolai V. Gnuchev, Vince Guerriero, Georgii P. Georgiev, Lidia P. Sashchenko

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Tag7 (PGRP-S) was described as an innate immunity protein. Earlier we have shown that Tag7 forms with Hsp70 a stable complex with cytotoxic and antitumor activity. The same complex is formed in and secreted by cytotoxic T-lymphocytes. We have also found that Hsp-binding protein HspBP1 incapacitates the Tag7-Hsp70 complex. Here we have studied the interaction of extracellular Tag7 and HspBP1. We have shown that HspBP1 binds Tag7 in the conditioned medium of tumor CSML0 cells, thereby preventing formation of the cytotoxic Tag7-Hsp70 complex. We have also found that Tag7, if present in serum (in every third donor on average), is always in complex with HspBP1. This may be a protective measure against indiscriminate attack of the cytotoxic complex on normal cells.

Original language	English (US)
Pages (from-to)	203-206
Number of pages	4
Journal	Biochimie
Volume	94
Issue number	1
DOIs	https://doi.org/10.1016/j.biochi.2011.10.007
State	Published - Jan 2012
Externally published	Yes

Keywords

Cytotoxity
HspBP1
Human serum
Tag7
Tag7-Hsp70 complex

ASJC Scopus subject areas

Biochemistry

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10.1016/j.biochi.2011.10.007

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Yashin, D. V., Dukhanina, E. A., Kabanova, O. D., Romanova, E. A., Lukyanova, T. I., Tonevitskii, A. G., Belogurov, A. A., Raynes, D. A., Sheludchenkov, A. A., Gnuchev, N. V., Guerriero, V., Georgiev, G. P., & Sashchenko, L. P. (2012). Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum. Biochimie, 94(1), 203-206. https://doi.org/10.1016/j.biochi.2011.10.007

Yashin, DV, Dukhanina, EA, Kabanova, OD, Romanova, EA, Lukyanova, TI, Tonevitskii, AG, Belogurov, AA, Raynes, DA, Sheludchenkov, AA, Gnuchev, NV, Guerriero, V, Georgiev, GP & Sashchenko, LP 2012, 'Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum', Biochimie, vol. 94, no. 1, pp. 203-206. https://doi.org/10.1016/j.biochi.2011.10.007

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title = "Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum",

abstract = "Tag7 (PGRP-S) was described as an innate immunity protein. Earlier we have shown that Tag7 forms with Hsp70 a stable complex with cytotoxic and antitumor activity. The same complex is formed in and secreted by cytotoxic T-lymphocytes. We have also found that Hsp-binding protein HspBP1 incapacitates the Tag7-Hsp70 complex. Here we have studied the interaction of extracellular Tag7 and HspBP1. We have shown that HspBP1 binds Tag7 in the conditioned medium of tumor CSML0 cells, thereby preventing formation of the cytotoxic Tag7-Hsp70 complex. We have also found that Tag7, if present in serum (in every third donor on average), is always in complex with HspBP1. This may be a protective measure against indiscriminate attack of the cytotoxic complex on normal cells.",

keywords = "Cytotoxity, HspBP1, Human serum, Tag7, Tag7-Hsp70 complex",

author = "Yashin, {Denis V.} and Dukhanina, {Elena A.} and Kabanova, {Olga D.} and Romanova, {Elena A.} and Lukyanova, {Tamara I.} and Tonevitskii, {Alexsander G.} and Belogurov, {Alexey A.} and Raynes, {Deborah A.} and Sheludchenkov, {Anton A.} and Gnuchev, {Nikolai V.} and Vince Guerriero and Georgiev, {Georgii P.} and Sashchenko, {Lidia P.}",

note = "Funding Information: This work was supported by the National Institute of General Medical Sciences grant number GM072628-02 , the Russian Foundation for Basic Research , RAS program for Molecular and Cellular Biology , International Centre for Genetic Engineering and Biotechnology (ICGEB) and by Federal special-purpose program “Scientific and educational personnel of innovative Russia” ",

year = "2012",

month = jan,

doi = "10.1016/j.biochi.2011.10.007",

language = "English (US)",

volume = "94",

pages = "203--206",

journal = "Biochimie",

issn = "0300-9084",

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T1 - Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum

AU - Yashin, Denis V.

AU - Dukhanina, Elena A.

AU - Kabanova, Olga D.

AU - Romanova, Elena A.

AU - Lukyanova, Tamara I.

AU - Tonevitskii, Alexsander G.

AU - Belogurov, Alexey A.

AU - Raynes, Deborah A.

AU - Sheludchenkov, Anton A.

AU - Gnuchev, Nikolai V.

AU - Guerriero, Vince

AU - Georgiev, Georgii P.

AU - Sashchenko, Lidia P.

N1 - Funding Information: This work was supported by the National Institute of General Medical Sciences grant number GM072628-02 , the Russian Foundation for Basic Research , RAS program for Molecular and Cellular Biology , International Centre for Genetic Engineering and Biotechnology (ICGEB) and by Federal special-purpose program “Scientific and educational personnel of innovative Russia”

PY - 2012/1

Y1 - 2012/1

N2 - Tag7 (PGRP-S) was described as an innate immunity protein. Earlier we have shown that Tag7 forms with Hsp70 a stable complex with cytotoxic and antitumor activity. The same complex is formed in and secreted by cytotoxic T-lymphocytes. We have also found that Hsp-binding protein HspBP1 incapacitates the Tag7-Hsp70 complex. Here we have studied the interaction of extracellular Tag7 and HspBP1. We have shown that HspBP1 binds Tag7 in the conditioned medium of tumor CSML0 cells, thereby preventing formation of the cytotoxic Tag7-Hsp70 complex. We have also found that Tag7, if present in serum (in every third donor on average), is always in complex with HspBP1. This may be a protective measure against indiscriminate attack of the cytotoxic complex on normal cells.

AB - Tag7 (PGRP-S) was described as an innate immunity protein. Earlier we have shown that Tag7 forms with Hsp70 a stable complex with cytotoxic and antitumor activity. The same complex is formed in and secreted by cytotoxic T-lymphocytes. We have also found that Hsp-binding protein HspBP1 incapacitates the Tag7-Hsp70 complex. Here we have studied the interaction of extracellular Tag7 and HspBP1. We have shown that HspBP1 binds Tag7 in the conditioned medium of tumor CSML0 cells, thereby preventing formation of the cytotoxic Tag7-Hsp70 complex. We have also found that Tag7, if present in serum (in every third donor on average), is always in complex with HspBP1. This may be a protective measure against indiscriminate attack of the cytotoxic complex on normal cells.

KW - Cytotoxity

KW - HspBP1

KW - Human serum

KW - Tag7

KW - Tag7-Hsp70 complex

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Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum

Abstract

Keywords

ASJC Scopus subject areas

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