Extracellular HspBP1 inhibits formation of a cytotoxic Tag7-Hsp70 complex in vitro and in human serum

Denis V. Yashin, Elena A. Dukhanina, Olga D. Kabanova, Elena A. Romanova, Tamara I. Lukyanova, Alexsander G. Tonevitskii, Alexey A. Belogurov, Deborah A. Raynes, Anton A. Sheludchenkov, Nikolai V. Gnuchev, Vince Guerriero, Georgii P. Georgiev, Lidia P. Sashchenko

    Research output: Contribution to journalArticlepeer-review

    4 Scopus citations

    Abstract

    Tag7 (PGRP-S) was described as an innate immunity protein. Earlier we have shown that Tag7 forms with Hsp70 a stable complex with cytotoxic and antitumor activity. The same complex is formed in and secreted by cytotoxic T-lymphocytes. We have also found that Hsp-binding protein HspBP1 incapacitates the Tag7-Hsp70 complex. Here we have studied the interaction of extracellular Tag7 and HspBP1. We have shown that HspBP1 binds Tag7 in the conditioned medium of tumor CSML0 cells, thereby preventing formation of the cytotoxic Tag7-Hsp70 complex. We have also found that Tag7, if present in serum (in every third donor on average), is always in complex with HspBP1. This may be a protective measure against indiscriminate attack of the cytotoxic complex on normal cells.

    Original languageEnglish (US)
    Pages (from-to)203-206
    Number of pages4
    JournalBiochimie
    Volume94
    Issue number1
    DOIs
    StatePublished - Jan 2012

    Keywords

    • Cytotoxity
    • HspBP1
    • Human serum
    • Tag7
    • Tag7-Hsp70 complex

    ASJC Scopus subject areas

    • Biochemistry

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