Abstract
Increasing the depth-of-field (DOF) while maintaining high resolution imaging has been a classical challenge for conventional microscopes. Extended DOF (EDOF) is especially essential for imaging thick specimens. We present a microscope capable of capturing EDOF images in a single shot. A volumetric optical sampling method is applied by rapidly scanning the focus of a vari-focal microscope objective throughout the extended depths of a thick specimen within the duration of a single detector exposure. An EDOF image is reconstructed by deconvolving the captured image with the response function of the system. Design of a varifocal objective and algorithms for restoring EDOF images are presented. Proof-of-concept experimental results demonstrate significantly extended DOF compared to the conventional microscope counterparts.
Original language | English (US) |
---|---|
Pages (from-to) | 353-362 |
Number of pages | 10 |
Journal | Optics Express |
Volume | 19 |
Issue number | 1 |
DOIs | |
State | Published - Jan 3 2011 |
ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Fingerprint
Dive into the research topics of 'Extended depth-of-field microscopic imaging with a variable focus microscope objective'. Together they form a unique fingerprint.Datasets
-
Extended depth-of-field microscopic imaging with a variable focus microscope objective
Liu, S. (Creator) & Hua, H. (Creator), figshare, 2011
DOI: 10.6084/m9.figshare.c.3763070.v1, https://figshare.com/collections/Extended_depth-of-field_microscopic_imaging_with_a_variable_focus_microscope_objective/3763070/1
Dataset
-
Extended depth-of-field microscopic imaging with a variable focus microscope objective
Liu, S. (Creator) & Hua, H. (Creator), figshare, 2011
DOI: 10.6084/m9.figshare.c.3763070, https://figshare.com/collections/Extended_depth-of-field_microscopic_imaging_with_a_variable_focus_microscope_objective/3763070
Dataset