Expression of the matrix metalloproteinase promatrilysin in coculture of prostate carcinoma cell lines

M. S. Stratton, H. Sirvent, T. S. Udayakumar, R. B. Nagle, G. T. Bowden

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


BACKGROUND. Matrix metalloproteinases (MMPs) are involved in tumor progression. Matrilysin (MMP-7) has been shown to be upregulated in prostatic carcinomas and can increase the invasive capacity of DU-145 cells. Because of the heterogenous nature of prostatic tumors, we examined promatrilysin expression in cocultures containing two different prostatic carcinoma cell lines, DU-145 and LNCaP. METHODS. Using enzyme linked immunosorbent assay (ELISA) analyses, promatrilysin expression was measured in DU-145/LNCaP cocultures and conditioned media cross-cultures. The effects of blocking IL-6 on promatrilysin expression were examined by pretreating conditioned media with IL-6 neutralizing antibody. RESULTS. A significant induction of promatrilysin expression was observed in DU-145/ LNCaP cocultures compared to LNCaP cells alone. In addition, DU-145 conditioned medium induced the same fold induction of promatrilysin as was observed in the cocultures. LNCaP cell conditioned medium did not induce promatrilysin expression in DU-145 cells. Neutralization of IL-6 with neutralizing antibody abrogated DU-145 conditioned media induced promatrilysin expression to baseline levels. CONCLUSIONS. IL-6 secreted by DU-145 cells can induce promatrilysin expression in LNCaP cells. IL-6, in vivo, may act as a paracrine signaling factor that regulates matrix metalloproteinase expression. Therefore, IL-6 may play a role in invasive metastatic processes of a prostate carcinoma.

Original languageEnglish (US)
Pages (from-to)206-209
Number of pages4
Issue number3
StatePublished - 2001


  • Coculture
  • DU-145
  • IL-6
  • LNCaP
  • Matrilysin
  • Prostate carcinoma

ASJC Scopus subject areas

  • Oncology
  • Urology


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