Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Kristi R. Harkins; Richard A. Jefferson; Tony A. Kavanagh; Michael W. Bevan; David W. Galbraith

doi:10.1073/pnas.87.2.816

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Kristi R. Harkins
, Richard A. Jefferson
, Tony A. Kavanagh
, Michael W. Bevan
, David W. Galbraith

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

Original language	English (US)
Pages (from-to)	816-820
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	87
Issue number	2
DOIs	https://doi.org/10.1073/pnas.87.2.816
State	Published - 1990

Keywords

Flow cytometry
Gene expression
Nicotiana tabacum
β-glucuronidase

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.87.2.816

Cite this

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. / Harkins, Kristi R.; Jefferson, Richard A.; Kavanagh, Tony A. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 2, 1990, p. 816-820.

Research output: Contribution to journal › Article › peer-review

@article{5cc7cd5be911432c999569a4ea7cedc6,

title = "Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts",

abstract = "We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.",

keywords = "Flow cytometry, Gene expression, Nicotiana tabacum, β-glucuronidase",

author = "Harkins, \{Kristi R.\} and Jefferson, \{Richard A.\} and Kavanagh, \{Tony A.\} and Bevan, \{Michael W.\} and Galbraith, \{David W.\}",

year = "1990",

doi = "10.1073/pnas.87.2.816",

language = "English (US)",

volume = "87",

pages = "816--820",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "2",

}

TY - JOUR

T1 - Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

AU - Harkins, Kristi R.

AU - Jefferson, Richard A.

AU - Kavanagh, Tony A.

AU - Bevan, Michael W.

AU - Galbraith, David W.

PY - 1990

Y1 - 1990

N2 - We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

AB - We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

KW - Flow cytometry

KW - Gene expression

KW - Nicotiana tabacum

KW - β-glucuronidase

UR - https://www.scopus.com/pages/publications/0025021833

UR - https://www.scopus.com/pages/publications/0025021833#tab=citedBy

U2 - 10.1073/pnas.87.2.816

DO - 10.1073/pnas.87.2.816

M3 - Article

C2 - 2300565

AN - SCOPUS:0025021833

SN - 0027-8424

VL - 87

SP - 816

EP - 820

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 2

ER -

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this