Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Kristi R. Harkins; Richard A. Jefferson; Tony A. Kavanagh; Michael W. Bevan; David W. Galbraith

doi:10.1073/pnas.87.2.816

Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

Kristi R. Harkins, Richard A. Jefferson, Tony A. Kavanagh, Michael W. Bevan, David W. Galbraith

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Abstract

We have analyzed the expression of chimeric genes in populations of protoplasts isolated from the photosynthetic and nonphotosynthetic tissues within leaves of transgenic tobacco plants and separated by fluorescence-activated cell sorting. Expression of transcriptional gene fusions controlled by promoters from photosynthesis-associated genes showed a striking dependence on cell type. These patterns of expression were preserved when the gene fusions were transfected into normal (nontransgenic) tobacco leaf protoplasts.

Original language	English (US)
Pages (from-to)	816-820
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	87
Issue number	2
DOIs	https://doi.org/10.1073/pnas.87.2.816
State	Published - 1990

Keywords

Flow cytometry
Gene expression
Nicotiana tabacum
β-glucuronidase

ASJC Scopus subject areas

General

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10.1073/pnas.87.2.816

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Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts. / Harkins, Kristi R.; Jefferson, Richard A.; Kavanagh, Tony A. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 2, 1990, p. 816-820.

Research output: Contribution to journal › Article › peer-review

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AU - Bevan, Michael W.

AU - Galbraith, David W.

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Expression of photosynthesis-related gene fusions is restricted by cell type in transgenic plants and in transfected protoplasts

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