Expression of mTOR signaling pathway markers in prostate cancer progression

Celeste L. Kremer, Rob R. Klein, Jenny Mendelson, Walden Browne, Linda K. Samadzedeh, Kristie Vanpatten, Lindsey Highstrom, Gary A. Pestano, Raymond B. Nagle

Research output: Contribution to journalArticlepeer-review

152 Scopus citations


BACKGROUND. The PI3K/AKT/mTOR pathway is central to prostate cancer progression. A preliminary investigation of immuno-histochemical expression of mammalian target of rapamycin (mTOR) pathway markers was undertaken to identify patterns of expression in prostate tissue. METHODS. Immunohistochemistry was performed on a custom-made prostate tissue array. Mean long scores and variability of long scores for each marker were recorded for normal lumenal cells, prostate intraepithelial neoplasia (PIN), and cancer. RESULTS. Expression of PTEN decreased and mTOR signaling pathway markers increased in PIN and in cancer as compared to normal cells in the majority of samples. Overexpression of 4E-BP1 and p-4E-BP1 was observed in PIN and cancer. However, in cancer, the overexpression of 4E-BP1 was significantly higher than with any other marker. DISCUSSION. Results suggest that 4E-BP1 overexpression is strongly associated with prostate cancer, especially when combined with PTEN and mTOR expression data. Hierarchical clustering analysis utilizing PTEN, mTOR, and 4E-BP1 separated normal from cancer cell populations in most cases.

Original languageEnglish (US)
Pages (from-to)1203-1212
Number of pages10
Issue number11
StatePublished - Aug 1 2006


  • 4E-BP1
  • PTEN
  • Prostate cancer
  • Prostate tissue array
  • mTOR

ASJC Scopus subject areas

  • Oncology
  • Urology


Dive into the research topics of 'Expression of mTOR signaling pathway markers in prostate cancer progression'. Together they form a unique fingerprint.

Cite this