Expression of miR-206 in human islets and its role in glucokinase regulation

Mugdha V. Joglekar, Wilson K.M. Wong, Cody Lee Maynard, Malati R. Umrani, David Martin, Thomas Loudovaris, Helen E. Thomas, Louise T. Dalgaard, Anandwardhan A. Hardikar

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Inappropriate insulin secretion from β-cells is considered as an early sign of impaired glucose tolerance and type 2 diabetes (T2D). Glucokinase (GCK) is an important enzyme that regulates glucose metabolism and ensures that the normal circulating glucose concentrations are maintained. GCK expression is induced by glucose and regulated via transcription factors and regulatory proteins. Recently, microRNA-206 (miR-206) was reported to regulate GCK and alter glucose tolerance in normal and high-fat diet-fed mice. Although the study findings have implications for human diabetes, studies in human islets are lacking. Here, we analyze human islets from individuals without or with T2D, using TaqMan-based real-time qPCR at the tissue (isolated islet) level as well as at single cell resolution, to assess the relationship between miR-206 and GCK expression in normal and T2D human islets. Our data suggest that, unlike mouse islets, human islets do not exhibit any correlation between miR-206 and GCK transcripts. These data implicate the need for further studies aimed toward exploring its potential role(s) in human islets.

Original languageEnglish (US)
Pages (from-to)E634-E637
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume315
Issue number4
DOIs
StatePublished - Oct 6 2018
Externally publishedYes

Keywords

  • Diabetes
  • Glucokinase
  • Human islets
  • microRNA
  • miR-206

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Physiology
  • Physiology (medical)

Fingerprint

Dive into the research topics of 'Expression of miR-206 in human islets and its role in glucokinase regulation'. Together they form a unique fingerprint.

Cite this