TY - JOUR
T1 - Expression of Distinct Classes of Titin Isoforms in Striated and Smooth Muscles by Alternative Splicing, and Their Conserved Interaction with Filamins
AU - Labeit, Siegfried
AU - Lahmers, Sunshine
AU - Burkart, Christoph
AU - Fong, Chi
AU - McNabb, Mark
AU - Witt, Stephanie
AU - Witt, Christian
AU - Labeit, Dietmar
AU - Granzier, Henk
N1 - Funding Information:
We are grateful to Dr Ralph Erber for invaluable advice on vascular histology, to the animal core facility at WSU for provision of porcine specimens, and Honghui Zhou for excellent technical assistance. This work was supported by the Deutsche Forschungsgemeinschaft (La1969/1-1 to D.L. and La668/9-1 to S.L.), and by the NIH (HL61487/HL62881 to H.G.).
PY - 2006/9/29
Y1 - 2006/9/29
N2 - While the role of titin as a sarcomeric protein is well established, its potential functional role(s) in smooth muscles and non-muscle tissues are controversial. We used a titin exon array to search for which part(s) of the human titin transcriptional unit encompassing 363 exons is(are) expressed in non-striated muscle tissues. Expression profiling of adult smooth muscle tissues (aorta, bladder, carotid, stomach) identified alternatively spliced titin isoforms, encompassing 80 to about 100 exons. These exons code for parts of the titin Z-disk, I-band and A-band regions, allowing the truncated smooth muscle titin isoform to link Z-disks/dense bodies together with thick filaments. Consistent with the array data, Western blot studies detected the expression of ∼1 MDa smooth muscle titin in adult smooth muscles, reacting with selected Z-disc, I-band, and A-band titin antibodies. Immunofluorescence with these antibodies located smooth muscle titin in the cytoplasm of cultured human aortic smooth muscle cells and in the tunica media of intact adult bovine aorta. Real time PCR studies suggested that smooth muscle titins are expressed from a promoter located 35 kb or more upstream of the transcription initiation site used for striated muscle titin, driving expression of a bi-cistronic mRNA, coding 5′ for the anonymous gene FL39502, followed 3′ by titin, respectively. Our work showed that smooth muscle and striated muscle titins share in their conserved amino-terminal regions binding sites for α-actinin and filamins: Yeast two-hybrid screens using Z2-Zis1 titin baits identified prey clones coding for α-actinin-1 and filamin-A from smooth muscle, and α-actinin-2/3, filamin-C, and nebulin from skeletal muscle cDNA libraries, respectively. This suggests that the titin Z2-Zis1 domain can link filamins and α-actinin together in the periphery of the Z-line/dense bodies in a fashion that is conserved in smooth and striated muscles.
AB - While the role of titin as a sarcomeric protein is well established, its potential functional role(s) in smooth muscles and non-muscle tissues are controversial. We used a titin exon array to search for which part(s) of the human titin transcriptional unit encompassing 363 exons is(are) expressed in non-striated muscle tissues. Expression profiling of adult smooth muscle tissues (aorta, bladder, carotid, stomach) identified alternatively spliced titin isoforms, encompassing 80 to about 100 exons. These exons code for parts of the titin Z-disk, I-band and A-band regions, allowing the truncated smooth muscle titin isoform to link Z-disks/dense bodies together with thick filaments. Consistent with the array data, Western blot studies detected the expression of ∼1 MDa smooth muscle titin in adult smooth muscles, reacting with selected Z-disc, I-band, and A-band titin antibodies. Immunofluorescence with these antibodies located smooth muscle titin in the cytoplasm of cultured human aortic smooth muscle cells and in the tunica media of intact adult bovine aorta. Real time PCR studies suggested that smooth muscle titins are expressed from a promoter located 35 kb or more upstream of the transcription initiation site used for striated muscle titin, driving expression of a bi-cistronic mRNA, coding 5′ for the anonymous gene FL39502, followed 3′ by titin, respectively. Our work showed that smooth muscle and striated muscle titins share in their conserved amino-terminal regions binding sites for α-actinin and filamins: Yeast two-hybrid screens using Z2-Zis1 titin baits identified prey clones coding for α-actinin-1 and filamin-A from smooth muscle, and α-actinin-2/3, filamin-C, and nebulin from skeletal muscle cDNA libraries, respectively. This suggests that the titin Z2-Zis1 domain can link filamins and α-actinin together in the periphery of the Z-line/dense bodies in a fashion that is conserved in smooth and striated muscles.
KW - Z-disks and dense bodies
KW - differential splicing
KW - filamins
KW - smooth and striated muscle
KW - titin
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U2 - 10.1016/j.jmb.2006.07.077
DO - 10.1016/j.jmb.2006.07.077
M3 - Article
C2 - 16949617
AN - SCOPUS:33748079591
SN - 0022-2836
VL - 362
SP - 664
EP - 681
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 4
ER -