Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase

Z. Khalpey; K. Kalsi; A. Yuen; J. Karbowska; Z. Kochan; E. M. Slominska; M. Forni; M. Bacci; M. Macherini; P. Batten; M. Lavitrano; M. H. Yacoub; R. T. Smolenski

doi:10.1081/NCN-59707

Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase

Z. Khalpey, K. Kalsi, A. Yuen, J. Karbowska, Z. Kochan, E. M. Slominska, M. Forni, M. Bacci, M. Macherini, P. Batten, M. Lavitrano, M. H. Yacoub, R. T. Smolenski

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Ecto-5′-nucleotidase (E5′ N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of ES′ N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5′ N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5′ N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of ESN decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.

Original language	English (US)
Pages (from-to)	271-274
Number of pages	4
Journal	Nucleosides, Nucleotides and Nucleic Acids
Volume	24
Issue number	4
DOIs	https://doi.org/10.1081/NCN-59707
State	Published - 2005
Externally published	Yes

Keywords

Complement
Ecto-5′-Nucleotidease
Endothelial cells
Nucleotides
Xenotransplantation

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Genetics

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10.1081/NCN-59707

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title = "Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase",

abstract = "Ecto-5′-nucleotidase (E5′ N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of ES′ N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5′ N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5′ N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of ESN decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.",

keywords = "Complement, Ecto-5′-Nucleotidease, Endothelial cells, Nucleotides, Xenotransplantation",

author = "Z. Khalpey and K. Kalsi and A. Yuen and J. Karbowska and Z. Kochan and Slominska, {E. M.} and M. Forni and M. Bacci and M. Macherini and P. Batten and M. Lavitrano and Yacoub, {M. H.} and Smolenski, {R. T.}",

note = "Funding Information: This study was supported by the British Heart Foundation (grant PG 99/173) and Magdi Yacoub Institute.",

year = "2005",

doi = "10.1081/NCN-59707",

language = "English (US)",

volume = "24",

pages = "271--274",

journal = "Nucleosides, Nucleotides and Nucleic Acids",

issn = "1525-7770",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase

AU - Khalpey, Z.

AU - Kalsi, K.

AU - Yuen, A.

AU - Karbowska, J.

AU - Kochan, Z.

AU - Slominska, E. M.

AU - Forni, M.

AU - Bacci, M.

AU - Macherini, M.

AU - Batten, P.

AU - Lavitrano, M.

AU - Yacoub, M. H.

AU - Smolenski, R. T.

N1 - Funding Information: This study was supported by the British Heart Foundation (grant PG 99/173) and Magdi Yacoub Institute.

PY - 2005

Y1 - 2005

N2 - Ecto-5′-nucleotidase (E5′ N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of ES′ N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5′ N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5′ N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of ESN decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.

AB - Ecto-5′-nucleotidase (E5′ N) is an extracellular enzyme forming anti-inflammatory and immunosuppressive adenosine. We evaluated whether confrontation of pig heart and endothelial cells with human blood changes the activity of ES′ N. Pig hearts were perfused ex vivo with fresh human blood for 4 h. Pig aortic endothelial cells (PAEC) were incubated in vitro with human plasma for 3 h. Ex vivo perfusion of pig heart with fresh human blood resulted in a decrease in E5′ N activity to 62% and 61% of initial in wild-type and transgenic pig hearts, respectively. PAEC activity of E5′ N decreased to 71% and 50% of initial after 3 h exposure to heat-inactivated and active complement human plasma, respectively, while it remained constant in controls. Pig heart activity of ESN decreased following exposure to human blood, which may affect adenosine production and exacerbate hyperacute and vascular rejection.

KW - Complement

KW - Ecto-5′-Nucleotidease

KW - Endothelial cells

KW - Nucleotides

KW - Xenotransplantation

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U2 - 10.1081/NCN-59707

DO - 10.1081/NCN-59707

M3 - Article

C2 - 16021914

AN - SCOPUS:22044451634

SN - 1525-7770

VL - 24

SP - 271

EP - 274

JO - Nucleosides, Nucleotides and Nucleic Acids

JF - Nucleosides, Nucleotides and Nucleic Acids

IS - 4

ER -

Exposure to human blood inactivates swine endothelial ecto-5′- nucleotidase

Abstract

Keywords

ASJC Scopus subject areas

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