Experimental validation of candidate schizophrenia gene ZNF804A as target for hsa-miR-137

Albert H. Kim, Erin K. Parker, Vernell Williamson, Gowon O. McMichael, Ayman H. Fanous, Vladimir I. Vladimirov

Research output: Contribution to journalArticlepeer-review

78 Scopus citations

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that mainly function as negative regulators of gene expression (Lai, 2002) and have been shown to be involved in schizophrenia etiology through genetic and expression studies (Burmistrova et al., 2007; Hansen et al., 2007a; Perkins et al., 2007; Beveridge et al., 2010; Kim et al., 2010). In a mega analysis of genome-wide association study (GWAS) of schizophrenia (SZ) and bipolar disorders (BP), a polymorphism (rs1625579) located in the primary transcript of a miRNA gene, hsa-miR-137, was reported to be strongly associated with SZ. Four SZ loci (CACNA1C, TCF4, CSMD1, C10orf26) achieving genome-wide significance in the same study were predicted and later experimentally validated (Kwon et al., 2011) as hsa-miR-137 targets. Here, using in silico, cellular and luciferase based approaches we also provide evidence that another well replicated candidate schizophrenia gene, ZNF804A, is also target for hsa-miR-137.

Original languageEnglish (US)
Pages (from-to)60-64
Number of pages5
JournalSchizophrenia Research
Volume141
Issue number1
DOIs
StatePublished - Oct 2012
Externally publishedYes

Keywords

  • GWAS
  • Gene expression
  • Luciferase
  • MiRNA
  • Real-time PCR
  • Schizophrenia

ASJC Scopus subject areas

  • Psychiatry and Mental health
  • Biological Psychiatry

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