TY - JOUR
T1 - Experimental validation of candidate schizophrenia gene ZNF804A as target for hsa-miR-137
AU - Kim, Albert H.
AU - Parker, Erin K.
AU - Williamson, Vernell
AU - McMichael, Gowon O.
AU - Fanous, Ayman H.
AU - Vladimirov, Vladimir I.
N1 - Funding Information:
Funding for this study was provided by SMRI grant (# 08R-1959 ) and Thomas Jeffress & Kate Miller Jeffress Memorial Trust ( J-1015 ) to V.I.V.; the funding bodies had no further role in study design; in the collection, analysis and interpretation of data; in the writing of the report; and in the decision to submit the paper for publication. Funding for A.H.F. was provided by a grant from the Department of Veterans Affairs Merit Review Program .
PY - 2012/10
Y1 - 2012/10
N2 - MicroRNAs (miRNAs) are small non-coding RNAs that mainly function as negative regulators of gene expression (Lai, 2002) and have been shown to be involved in schizophrenia etiology through genetic and expression studies (Burmistrova et al., 2007; Hansen et al., 2007a; Perkins et al., 2007; Beveridge et al., 2010; Kim et al., 2010). In a mega analysis of genome-wide association study (GWAS) of schizophrenia (SZ) and bipolar disorders (BP), a polymorphism (rs1625579) located in the primary transcript of a miRNA gene, hsa-miR-137, was reported to be strongly associated with SZ. Four SZ loci (CACNA1C, TCF4, CSMD1, C10orf26) achieving genome-wide significance in the same study were predicted and later experimentally validated (Kwon et al., 2011) as hsa-miR-137 targets. Here, using in silico, cellular and luciferase based approaches we also provide evidence that another well replicated candidate schizophrenia gene, ZNF804A, is also target for hsa-miR-137.
AB - MicroRNAs (miRNAs) are small non-coding RNAs that mainly function as negative regulators of gene expression (Lai, 2002) and have been shown to be involved in schizophrenia etiology through genetic and expression studies (Burmistrova et al., 2007; Hansen et al., 2007a; Perkins et al., 2007; Beveridge et al., 2010; Kim et al., 2010). In a mega analysis of genome-wide association study (GWAS) of schizophrenia (SZ) and bipolar disorders (BP), a polymorphism (rs1625579) located in the primary transcript of a miRNA gene, hsa-miR-137, was reported to be strongly associated with SZ. Four SZ loci (CACNA1C, TCF4, CSMD1, C10orf26) achieving genome-wide significance in the same study were predicted and later experimentally validated (Kwon et al., 2011) as hsa-miR-137 targets. Here, using in silico, cellular and luciferase based approaches we also provide evidence that another well replicated candidate schizophrenia gene, ZNF804A, is also target for hsa-miR-137.
KW - GWAS
KW - Gene expression
KW - Luciferase
KW - MiRNA
KW - Real-time PCR
KW - Schizophrenia
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U2 - 10.1016/j.schres.2012.06.038
DO - 10.1016/j.schres.2012.06.038
M3 - Article
C2 - 22883350
AN - SCOPUS:84865985475
SN - 0920-9964
VL - 141
SP - 60
EP - 64
JO - Schizophrenia Research
JF - Schizophrenia Research
IS - 1
ER -