TY - JOUR
T1 - Experimental characterization of methanol-acetic acid fixative sessile drop dynamics in dry and humid air by video imaging and interference analysis
AU - Gu, J.
AU - Zenhausern, F.
N1 - Funding Information:
The authors would like to thank Kevin Tao, a recipient of the Helios Scholarship, for help on initial experimental setup and preliminary results. The authors are also grateful to the Helios Education Foundation for its financial support.
PY - 2014/5/1
Y1 - 2014/5/1
N2 - Dynamics of methanol and acetic acid (3:1, v/v) fixative sessile drop is important for metaphase chromosomal spreads in cytogenetic assays. However, it has not been well characterized by biologists from a physical science point of view. In this work, a simple optical setup was built to record the fixative drop spreading and evaporation process. Drop film thickness, cross-sectional profile and surface thinning speed were constructed from the observed interference patterns to show evolution of the process in both dry and humid air. Surface thinning speed analysis at the drop center suggested different evaporation regimes. The ability of characterizing fluid behavior at a scale comparable to the size of cells by interference fringes is expected to facilitate further understanding of the metaphase spreading process at micro- and nano-scale.
AB - Dynamics of methanol and acetic acid (3:1, v/v) fixative sessile drop is important for metaphase chromosomal spreads in cytogenetic assays. However, it has not been well characterized by biologists from a physical science point of view. In this work, a simple optical setup was built to record the fixative drop spreading and evaporation process. Drop film thickness, cross-sectional profile and surface thinning speed were constructed from the observed interference patterns to show evolution of the process in both dry and humid air. Surface thinning speed analysis at the drop center suggested different evaporation regimes. The ability of characterizing fluid behavior at a scale comparable to the size of cells by interference fringes is expected to facilitate further understanding of the metaphase spreading process at micro- and nano-scale.
KW - Cytogenetic assay
KW - Interference analysis
KW - Marangoni effect
KW - Metaphase spread
KW - Methanol-acetic acid sessile drop
KW - Spreading and evaporation
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U2 - 10.1016/j.colsurfa.2014.02.047
DO - 10.1016/j.colsurfa.2014.02.047
M3 - Article
AN - SCOPUS:84896093468
SN - 0927-7757
VL - 449
SP - 141
EP - 147
JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects
JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects
IS - 1
ER -