Exon trapping: A genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA

Geoffrey M. Duyk; Suwon Kim; Richard M. Myers; David R. Cox

doi:10.1073/pnas.87.22.8995

Exon trapping: A genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA

Geoffrey M. Duyk, Suwon Kim, Richard M. Myers, David R. Cox

Research output: Contribution to journal › Article › peer-review

183 Scopus citations

Abstract

Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this 'exon trapping' strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened ≃20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.

Original language	English (US)
Pages (from-to)	8995-8999
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	87
Issue number	22
DOIs	https://doi.org/10.1073/pnas.87.22.8995
State	Published - 1990
Externally published	Yes

Keywords

RNA splicing
human genetics
retroviral vectors

ASJC Scopus subject areas

General

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abstract = "Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this 'exon trapping' strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened ≃20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.",

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author = "Duyk, {Geoffrey M.} and Suwon Kim and Myers, {Richard M.} and Cox, {David R.}",

year = "1990",

doi = "10.1073/pnas.87.22.8995",

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T1 - Exon trapping

T2 - A genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA

AU - Duyk, Geoffrey M.

AU - Kim, Suwon

AU - Myers, Richard M.

AU - Cox, David R.

PY - 1990

Y1 - 1990

N2 - Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this 'exon trapping' strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened ≃20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.

AB - Identification and recovery of transcribed sequences from cloned mammalian genomic DNA remains an important problem in isolating genes on the basis of their chromosomal location. We have developed a strategy that facilitates the recovery of exons from random pieces of cloned genomic DNA. The basis of this 'exon trapping' strategy is that, during a retroviral life cycle, genomic sequences of nonviral origin are correctly spliced and may be recovered as a cDNA copy of the introduced segment. By using this genetic assay for cis-acting sequences required for RNA splicing, we have screened ≃20 kilobase pairs of cloned genomic DNA and have recovered all four predicted exons.

KW - RNA splicing

KW - human genetics

KW - retroviral vectors

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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Exon trapping: A genetic screen to identify candidate transcribed sequences in cloned mammalian genomic DNA

Abstract

Keywords

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