Exercise-induced B cell mobilisation: Preliminary evidence for an influx of immature cells into the bloodstream

J. E. Turner, G. Spielmann, A. J. Wadley, S. Aldred, R. J. Simpson, J. P. Campbell

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The number of peripheral blood B lymphocytes doubles during acute exercise, but the phenotypic composition of this response remains unknown. In two independent exercise studies, using complimentary phenotyping strategies, we investigated the mobilisation patterns of distinct B cell subsets. In study one, nine healthy males (mean ± SD age: 22.1 ± 3.4 years) completed a continuous cycling bout at 80% V̇O2MAX for 20 min. In study two, seven healthy experienced cyclists (mean ± SD age: 29.9 ± 4.7 years) completed a 30 min cycling trial at a workload corresponding to + 5% of the individual blood lactate threshold. In study one, CD3 − CD19+ B cell subsets were classified into immature (CD27− CD10+), naïve (CD27− CD10−), memory (CD27+ CD38−), plasma cells/plasmablasts (CD27+ CD38+) and finally, recently purported ‘B1’ cells (CD27+ CD43+ CD69−). In study two, CD20+ B cells were classified into immature (CD27− IgD−), naïve (CD27− IgD+), and IgM+/IgG+/IgA+ memory cells (CD27+ IgD−). Total B cells exhibited a mean increase of 88% (study one) and 60% (study two) during exercise. In both studies, immature cells displayed the greatest increase, followed by memory cells, then naïve cells (study one: immature 130% > mature 105% > naïve 84%; study two: immature 110% > mature 56% > naïve 38%). Our findings show that, unlike T cells and NK cells, B cell mobilisation is not driven by effector status, and, for the first time, that B cell mobilisation during exercise is comprised of immature CD27− IgD−/CD10+ cells.

Original languageEnglish (US)
Pages (from-to)376-382
Number of pages7
JournalPhysiology and Behavior
Volume164
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Keywords

  • B lymphocytes
  • Exercise
  • Homing
  • Immune-surveillance
  • Lymphocytosis

ASJC Scopus subject areas

  • Experimental and Cognitive Psychology
  • Behavioral Neuroscience

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